| RRC ID |
74959
|
| 著者 |
Eun-Seo Cho, Seungjae Han, Kang-Han Lee, Cheol-Hee Kim, Young-Gyu Yoon
|
| タイトル |
3DM: deep decomposition and deconvolution microscopy for rapid neural activity imaging
|
| ジャーナル |
Optics Express
|
| Abstract |
We report the development of deep decomposition and deconvolution microscopy (3DM), a computational microscopy method for the volumetric imaging of neural activity. 3DM overcomes the major challenge of deconvolution microscopy, the ill-posed inverse problem. We take advantage of the temporal sparsity of neural activity to reformulate and solve the inverse problem using two neural networks which perform sparse decomposition and deconvolution. We demonstrate the capability of 3DM via in vivo imaging of the neural activity of a whole larval zebrafish brain with a field of view of 1040 µm × 400 µm × 235 µm and with estimated lateral and axial resolutions of 1.7 µm and 5.4 µm, respectively, at imaging rates of up to 4.2 volumes per second.
|
| 巻・号 |
29
|
| ページ |
32700
|
| 公開日 |
2021-9-27
|
| DOI |
10.1364/oe.439619
|
| PMID |
34615335
|
| MeSH |
Animals
Brain / diagnostic imaging*
Brain / physiology
Imaging, Three-Dimensional / methods*
Intravital Microscopy / methods
Larva
Microscopy, Confocal
Microscopy, Fluorescence / methods*
Neural Networks, Computer
Neurons / physiology
Optical Imaging / methods*
Spinal Cord / diagnostic imaging
Spinal Cord / physiology
Zebrafish / physiology*
|
| IF |
3.669
|
| リソース情報 |
| ゼブラフィッシュ |
UAS:GCaMP7a |
