RRC ID 75001
Author Venditti M, Pedalino C, Rosello M, Fasano G, Serafini M, Revenu C, Del Bene F, Tartaglia M, Lauri A.
Title A minimally invasive fin scratching protocol for fast genotyping and early selection of zebrafish embryos.
Journal Sci Rep
Abstract Current genetic modification and phenotyping methods in teleost fish allow detailed investigation of vertebrate mechanisms of development, modeling of specific aspects of human diseases and efficient testing of drugs at an organ/organismal level in an unparalleled fast and large-scale mode. Fish-based experimental approaches have boosted the in vivo verification and implementation of scientific advances, offering the quality guaranteed by animal models that ultimately benefit human health, and are not yet fully replaceable by even the most sophisticated in vitro alternatives. Thanks to highly efficient and constantly advancing genetic engineering as well as non-invasive phenotyping methods, the small zebrafish is quickly becoming a popular alternative to large animals' experimentation. This approach is commonly associated to invasive procedures and increased burden. Here, we present a rapid and minimally invasive method to obtain sufficient genomic material from single zebrafish embryos by simple and precise tail fin scratching that can be robustly used for at least two rounds of genotyping already from embryos within 48 h of development. The described protocol betters currently available methods (such as fin clipping), by minimizing the relative animal distress associated with biopsy at later or adult stages. It allows early selection of embryos with desired genotypes for strategizing culturing or genotype-phenotype correlation experiments, resulting in a net reduction of "surplus" animals used for mutant line generation.
Volume 12(1)
Pages 22597
Published 2022-12-30
DOI 10.1038/s41598-022-26822-7
PII 10.1038/s41598-022-26822-7
PMID 36585409
PMC PMC9803660
MeSH Animals Biopsy Genetic Engineering* Genotype Humans Models, Animal Zebrafish* / genetics
IF 3.998
Zebrafish SAGFF73A UAS:Gtuba2A