論文 - 詳細
| RRC ID | 75124 |
|---|---|
| 著者 | Ortiz de la Rosa JM, Nordmann P, Zong Z, Poirel L. |
| タイトル | Aliidiomarina shirensis as Possible Source of the Integron- and Plasmid-Mediated Fosfomycin Resistance Gene fosC2. |
| ジャーナル | Antimicrob Agents Chemother |
| Abstract |
In-silico analysis and cloning experiments identified a fosC2-like fosfomycin resistance gene in the chromosome of Aliidiomarina shirensis, with our data suggesting that this bacterium might be added to the list of species identified as reservoirs of fos-like genes that were subsequently acquired by other Gram-negative species. Indeed, the fosC2 gene was identified as acquired in Providencia huaxinensis and Aeromonas hydrophila isolates, with this gene being located in class 1 integron structures in the latter cases. Biochemical characterization and site-directed mutagenesis showed a higher catalytic efficiency for the intrinsic FosC2AS (from A. shirensis) than for the acquired FosC2 (from P. huaxinensis) enzyme due to a single substitution in the amino acid sequence (Gly43Glu). Notably, this study constitutes the first identification of the likely natural reservoir of a complete gene cassette (including its attC site). |
| 巻・号 | 66(3) |
| ページ | e0222721 |
| 公開日 | 2022-3-15 |
| DOI | 10.1128/aac.02227-21 |
| PMID | 35041510 |
| PMC | PMC8923222 |
| MeSH | Anti-Bacterial Agents / pharmacology Fosfomycin* / pharmacology Gammaproteobacteria Integrons / genetics Microbial Sensitivity Tests Plasmids / genetics Polymerase Chain Reaction |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 17761 |