| RRC ID |
75331
|
| 著者 |
Yang G, Li Y, Lin A, Zhuang L.
|
| タイトル |
Geobacter benzoatilyticus sp. nov., a novel benzoate-oxidizing, iron-reducing bacterium isolated from petroleum contaminated soil.
|
| ジャーナル |
Int J Syst Evol Microbiol
|
| Abstract |
A strictly anaerobic bacterial strain, designated Jerry-YXT, was isolated from petroleum-contaminated soil sampled in China. Strain Jerry-YXT was a Gram-stain-negative bacterium forming reddish colonies. It grew optimally at 30 °C and pH 7.0, and tolerated 1.0 % (w/v) NaCl. Strain Jerry-YXT was able to use fumarate, ferric citrate and ferrihydrite as electron acceptors, and ethanol, acetate and benzoate as electron donors. The major fatty acids of this strain were C16 : 0 and C16 : 1 ω7c/C16 : 1 ω6c (summed feature 3). The 16S rRNA gene sequence-based phylogenetic analysis placed this strain in the genus Geobacter, being most closely related to Geobacter metallireducens (98.2 % similarity), Geobacter hydrogenophilus (98.1 %) and Geobacter grbiciae (98.0 %). The DNA G+C content was 57.6 mol%. The average nucleotide identity and digital DNA-DNA hybridization values between the genomes of strain Jerry-YXT and G. metallireducens GS-15T were 81.8 and 35.4 %, respectively. The results of the polyphasic study allowed the genotypic and phenotypic differentiation of strain Jerry-YXT from its closest species, which suggested that strain Jerry-YXT represents a novel species of the genus Geobacter. The name for the proposed new species is Geobacter benzoatilyticus sp. nov. The type strain is Jerry-YXT (=MCCC 1K05659T=JCM 39190T).
|
| 巻・号 |
72(3)
|
| 公開日 |
2022-3-1
|
| DOI |
10.1099/ijsem.0.005281
|
| PMID |
35254233
|
| MeSH |
Bacterial Typing Techniques
Base Composition
Benzoates
DNA, Bacterial / genetics
Fatty Acids / chemistry
Geobacter* / genetics
Iron
Oxidation-Reduction
Petroleum*
Phylogeny
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Soil
|
| リソース情報 |
| 一般微生物 |
JCM 39190 |
