| RRC ID |
75346
|
| Author |
Wen Y, Zhang G, Bahadur A, Liu Y, Zhang Z, Chen T, Liu G, Zhang W.
|
| Title |
Streptomyces gobiensis sp. nov., an antimicrobial producing actinobacterium isolated from soil under black Gobi rocks.
|
| Journal |
Int J Syst Evol Microbiol
|
| Abstract |
A novel actinomycete, strain 1_25T, was isolated from soil under a black Gobi rock sample from Shuangta, PR China, and characterized using a polyphasic taxonomic approach. The results of comparative analysis of the 16S rRNA gene sequences indicated the 1_25T represented a member of the genus Streptomyces. Chemotaxonomic data revealed that 1_25T possessed MK-9(H8) as the major menaquinone. The cell wall contained ll-diaminopimelic acid (ll-DAP) and the whole-cell sugar pattern consisted of ribose, glucose and galactose. Major fatty acid methyl esters were observed to be iso-C16 : 0 (23.6 %), and anteiso-C15 : 0 (10.4 %). The genomic DNA G+C content of 1_25T was 69 mol %. The results of phylogenetic analysis based on 16S rRNA gene sequence indicated that 1_25T had high sequence similarity with Streptomyces qinglanensis 172205T (98.1 %), Streptomyces lycii TRM 66187T (98 %), and Streptomyces griseocarneus JCM4580T (98 %). In addition to the differences in phenotypic characters, the average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between 1_25T and closely related species were below the recommended threshold values for assigning strains to the same species. The fermentation product of 1_25T in ISP2 had an inhibitory effect on Staphylococcus aureus. On the basis of these genotypic and phenotypic characteristics, strain 1_25T (=JCM 34936T=GDMCC 4.216T) represents a novel species of the genus Streptomyces, for which the name Streptomyces gobiensis sp. nov. is proposed.
|
| Volume |
72(3)
|
| Published |
2022-3-1
|
| DOI |
10.1099/ijsem.0.005318
|
| PMID |
35320067
|
| MeSH |
Actinobacteria* / genetics
Anti-Infective Agents*
Bacterial Typing Techniques
Base Composition
DNA, Bacterial / genetics
Fatty Acids / chemistry
Phospholipids
Phylogeny
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Soil
Soil Microbiology
Streptomyces*
|
| Resource |
| General Microbes |
JCM 34936
JCM 4580 |
