論文 - 詳細
| RRC ID | 75375 |
|---|---|
| 著者 | Kweon OJ, Kim KH, Kim HM, Khan SA, Jeong JH, Lim YK, Kim HR, Jeon CO, Lee MK. |
| タイトル | Arsenicicoccus cauae sp. nov., isolated from the blood of a pediatric gastroenteritis patient. |
| ジャーナル | Int J Syst Evol Microbiol |
| Abstract |
A Gram-stain-positive coccus was isolated from the blood of a paediatric patient suffering from gastroenteritis. The taxonomic position of this catalase-positive, non-motile, non-spore-forming facultative anaerobe designated as strain MKL-02T was investigated using a polyphasic approach. Colonies grown on tryptic soy agar with 10 % sheep blood were circular, creamy yellow, and convex. Phylogenetic analysis based on 16S rRNA gene and whole-genome sequences revealed that this strain was most closely related to Arsenicicoccus bolidensis CCUG 47306T within the cluster of the genus Arsenicicoccus. Average nucleotide identity and digital DNA-DNA hybridization values between strain MKL-02T and A. bolidensis DSM 15745T, A. dermatophillus DSM 25571T and A. piscis DSM 22760T were 89.5 and 37.0 %, 79.6 and 22.4 %, and 75.9 and 21.0 %, respectively. The genomic size of strain MKL-02T was 3 423 857 bp with a 72.7 mol% G+C content. Growth was observed at 10-45 °C (optimum, 37-40 °C) and pH 6.0-10.0 (optimum, pH 7.0), in the presence of 0-10 % (w/v) NaCl (optimum, 0.5 %). Cells of strain MKL-02T were non-motile cocci and 0.50-0.60 µm long, as determined by transmission electron microscopy. The strain was catalase-positive and oxidase-negative. The major fatty acid type (>10 % of total) was C15 : 0. The polar lipid profile consisted of two unidentified phospholipids, three unidentified lipids and an unidentified aminophospholipid. The strain contained MK-8 (H4) as the predominant menaquinone. Based on phylogenetic and phenotypic considerations, it is proposed that strain MKL-02T be classified as a new species, named Arsenicicoccus cauae sp. nov. The type strain is MKL-02T (=NCCP 16967T=JCM 34624T). |
| 巻・号 | 72(5) |
| 公開日 | 2022-5-1 |
| DOI | 10.1099/ijsem.0.005381 |
| PMID | 35580017 |
| MeSH | Actinomycetales* / isolation & purification Actinomycetales Infections* / blood Actinomycetales Infections* / microbiology Animals Bacterial Typing Techniques Base Composition Catalase / genetics Child DNA, Bacterial / genetics Fatty Acids / chemistry Gastroenteritis* / blood Gastroenteritis* / microbiology Humans Nucleic Acid Hybridization Phospholipids / chemistry Phylogeny RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Sheep |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 10 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 34624 |