| RRC ID |
75426
|
| 著者 |
Mei N, Postec A, Bartoli M, Vandecasteele C, Wils L, Gil L, Monnin C, Pelletier B, Erauso G, Quéméneur M.
|
| タイトル |
Methanobacterium alkalithermotolerans sp. nov., a novel alkaliphilic and hydrogen-utilizing methanogen isolated from an alkaline geothermal spring (La Crouen, New Caledonia).
|
| ジャーナル |
Int J Syst Evol Microbiol
|
| Abstract |
An anaerobic, hydrogenotrophic methane-producing archaeon was isolated from an alkaline thermal spring (42 °C, pH 9.0) in New Caledonia. This methanogen, designated strain CANT, is alkaliphilic, thermotolerant, with Gram-positive staining non-motile cells. Strain CANT grows autotrophically using hydrogen exclusively as an energy source and carbon dioxide as the sole carbon source (without the requirement of yeast extract or other organic compounds). It grows at 20-45 °C (optimum, 45 °C) and pH 7.3-9.7 (optimum, pH 9.0). NaCl is not required for growth (optimum 0 %) but is tolerated up to 1.5 %. It resists novobiocin, streptomycin and vancomycin but is inhibited by ampicillin and penicillin, among other antibiotics. The genome consists of a circular chromosome (2.2 Mb) containing 2126 predicted protein-encoding genes with a G+C content of 36.4 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain CANT is a member of the genus Methanobacterium, most closely related to the alkaliphilic Methanobacterium alcaliphilum WeN4T with 98.5 % 16S rRNA gene sequence identity. The genomes of strain CANT and M. alcaliphilum DSM 3459, sequenced in this study, share 71.6 % average nucleotide identity and 14.0 % digital DNA-DNA hybridization. Therefore, phylogenetic and physiological results indicate that strain CANT represents a novel species, for which the name Methanobacterium alkalithermotolerans sp. nov. is proposed, and strain CANT (=DSM 102889T= JCM 31304T) is assigned as the type strain.
|
| 巻・号 |
72(10)
|
| 公開日 |
2022-10-1
|
| DOI |
10.1099/ijsem.0.005554
|
| PMID |
36260502
|
| MeSH |
Ampicillin
Anti-Bacterial Agents
Bacterial Typing Techniques
Base Composition
Carbon Dioxide
DNA, Bacterial / genetics
Fatty Acids / chemistry
Hot Springs*
Hydrogen
Methane
Methanobacterium* / genetics
New Caledonia
Novobiocin
Nucleotides
Penicillins
Phylogeny
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Sodium Chloride
Streptomycin
Vancomycin
|
| リソース情報 |
| 一般微生物 |
JCM 31304 |
