論文 - 詳細
| RRC ID | 75471 |
|---|---|
| 著者 | Chen Y, Wang M, Wang W, Zeng Y, Fan X, Tu M, Ma Y, Wei D. |
| タイトル | Metabolic Engineering for the Comprehensive Utilization of N,N-Dimethylformamide-Containing Wastewater. |
| ジャーナル | J Agric Food Chem |
| Abstract |
N, N-dimethylformamide is frequently present in industrial wastewater and is environmentally detrimental. The current study aims to assess the utilization and biodegradation of N, N-dimethylformamide-containing wastewater to lessen the associated environmental load. Results show that addition of wastewater containing N, N-dimethylformamide to Trichoderma reesei fermentation media enhances cellulase production and facilitates cellulose hydrolysis. However, N, N-dimethylformamide is a cellulase enhancer that is not degraded during cellulase production in T. reesei fermentation and is retained in the N, N-dimethylformamide-enhanced cellulase solution. Indeed, the cellulosic sugar solution generated via lignocellulose hydrolysis with N, N-dimethylformamide-enhanced cellulase retains N, N-dimethylformamide. We further identified three core enzyme modules─N, N-dimethylformamidase, dimethylamine dehydrogenase, and methylamine dehydrogenase enzyme─which were inserted into Escherichia coli to develop metabolically engineered strains. These strains degraded N, N-dimethylformamide and produced succinate using N, N-dimethylformamide-enhanced cellulosic sugar as the substrate. The platform described here can be applied to effectively convert waste into valuable bioproducts. |
| 巻・号 | 70(42) |
| ページ | 13574-13582 |
| 公開日 | 2022-10-26 |
| DOI | 10.1021/acs.jafc.2c05240 |
| PMID | 36223298 |
| MeSH | Carbohydrates Cellulase* / metabolism Cellulose / metabolism Dimethylformamide / metabolism Fermentation Hydrolysis Metabolic Engineering Succinic Acid / metabolism Sugars / metabolism Trichoderma* / metabolism Wastewater |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 7686 |