| RRC ID |
75508
|
| 著者 |
Zhang DF, Yao YF, Xue HP, Fu ZY, Zhang XM, Shao Z.
|
| タイトル |
Characterization of Marinilongibacter aquaticus gen. nov., sp. nov., a unique marine bacterium harboring four CRISPR-Cas systems in the phylum Bacteroidota.
|
| ジャーナル |
J Microbiol
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| Abstract |
A novel bacterium, designated YYF0007T, was isolated from an agar-degrading co-culture. The strain was found harboring four CRISPR-Cas systems of two classes in the chromosome and subsequently subjected to a study on polyphasic taxonomy. Pairwise analyses of the 16S rRNA gene sequences indicated that strain YYF0007T had highest 16S rRNA gene sequence similarity (92.2%) to Jiulongibacter sediminis JN-14-9T. The phylogenomic trees based on the 16S rRNA gene and 269 single-copy orthologous gene clusters (OCs) indicated that strain YYF0007T should be recognized as a novel genus of the family Spirosomaceae. The cells were Gramstain-negative, nonmotile, strictly aerobic, and straight long rods with no flagellum. Optimum growth occurred at 28°C and pH 7.0 with the presence of NaCl concentration 1.0-3.0% (w/v). The strain showed oxidase and catalase activities. The major fatty acids were C16:1ω5c, iso-C15:0 and summed feature 3 (C16:1ω7c and/or C16:1ω6c). The predominant isoprenoid quinone was MK-7. The complete genome size was 4.64 Mb with a DNA G + C content of 44.4%. Further typing of CRISPR-Cas systems in the family Spirosomaceae and the phylum Bacteroidota indicated that it was remarkable for strain YYF0007T featured by such a set of CRISPR-Cas systems. This trait highlights the applications of strain YYF-0007T in studies on the evolutionary dynamics and bacterial autoimmunity of CRISPR-Cas system as a potential model. The name Marinilongibacter aquaticus gen. nov., sp. nov. is proposed, and the type strain is YYF0007T (= MCCC 1K06017T = GDMCC 1.2428T = JCM 34683T).
|
| 巻・号 |
60(9)
|
| ページ |
905-915
|
| 公開日 |
2022-9-1
|
| DOI |
10.1007/s12275-022-2102-3
|
| PII |
10.1007/s12275-022-2102-3
|
| PMID |
35913596
|
| MeSH |
Bacterial Typing Techniques
Bacteroidetes* / genetics
CRISPR-Cas Systems*
DNA, Bacterial / genetics
Fatty Acids / chemistry
Phospholipids / chemistry
Phylogeny
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Ubiquinone / chemistry
|
| リソース情報 |
| 一般微生物 |
JCM 34683 |
