RRC ID 75734
Author McCallum S, Obata Y, Fourli E, Boeing S, Peddie CJ, Xu Q, Horswell S, Kelsh RN, Collinson L, Wilkinson D, Pin C, Pachnis V, Heanue TA.
Title Enteric glia as a source of neural progenitors in adult zebrafish.
Journal Elife
Abstract The presence and identity of neural progenitors in the enteric nervous system (ENS) of vertebrates is a matter of intense debate. Here, we demonstrate that the non-neuronal ENS cell compartment of teleosts shares molecular and morphological characteristics with mammalian enteric glia but cannot be identified by the expression of canonical glial markers. However, unlike their mammalian counterparts, which are generally quiescent and do not undergo neuronal differentiation during homeostasis, we show that a relatively high proportion of zebrafish enteric glia proliferate under physiological conditions giving rise to progeny that differentiate into enteric neurons. We also provide evidence that, similar to brain neural stem cells, the activation and neuronal differentiation of enteric glia are regulated by Notch signalling. Our experiments reveal remarkable similarities between enteric glia and brain neural stem cells in teleosts and open new possibilities for use of mammalian enteric glia as a potential source of neurons to restore the activity of intestinal neural circuits compromised by injury or disease.
Volume 9
Published 2020-8-27
DOI 10.7554/eLife.56086
PII 56086
PMID 32851974
PMC PMC7521928
MeSH Animals Brain / cytology Enteric Nervous System / cytology* Mice Neural Stem Cells / cytology Neuroglia / cytology* Receptors, Notch / metabolism Signal Transduction / physiology Zebrafish
IF 7.08
Zebrafish SAGFF(LF)234A, UAS:GFP, SAGFF(LF)217B