RRC ID |
75760
|
著者 |
Kasayama S, Sumitani S, Tanaka A, Yamanishi H, Nakamura N, Matsumoto K, Sato B.
|
タイトル |
Heparin inhibits autocrine stimulation but not fibroblast growth factor stimulation of cell proliferation of androgen-responsive Shionogi carcinoma 115.
|
ジャーナル |
J Cell Physiol
|
Abstract |
An androgen-responsive cloned cell line (SC-3) derived from Shionogi carcinoma 115 (SC115) has been shown to secrete fibroblast growth factor (FGF)-like peptide in response to androgen, which binds to FGF receptor and promotes the proliferation of SC-3 cells in an autocrine mechanism. Since the androgen-induced autocrine factor has a property to bind heparin, we examined the effects of heparin on the growth of SC-3 cells. Heparin was found to exhibit significant inhibition of testosterone-induced growth in a concentration-dependent manner: Approximately 50% inhibition was found at a concentration of 0.1 micrograms/ml. DNA synthesis of SC-3 cells induced by testosterone was also inhibited strongly by heparin, and less strongly by heparan sulfate and dermatan sulfate. Proliferation of SC-3 cells induced by acidic (a) or basic (b) FGF appeared not to be modulated by heparin. In contrast, heparin efficiently blocked DNA synthesis stimulated with androgen-induced growth factor in the conditioned medium from testosterone-treated cells. These results indicate that heparin inhibits autocrine loop in SC-3 cells induced by androgen. Thus, the autocrine growth factor possesses a different characteristic from aFGF and bFGF in that its bioactivities are negatively modulated by the glycosaminoglycan.
|
巻・号 |
148(2)
|
ページ |
260-6
|
公開日 |
1991-8-1
|
DOI |
10.1002/jcp.1041480211
|
PMID |
1715343
|
MeSH |
Animals
Cell Division / drug effects*
Cell Line
Culture Media
DNA, Neoplasm / biosynthesis
Fibroblast Growth Factor 1 / pharmacology
Fibroblast Growth Factor 2 / pharmacology
Fibroblast Growth Factors / pharmacology*
Growth Substances / pharmacology*
Heparin / pharmacology*
Testosterone / pharmacology*
Tumor Cells, Cultured
|
IF |
5.546
|
リソース情報 |
ヒト・動物細胞 |
NIH3T3-3(RCB0150) |