論文 - 詳細
| RRC ID | 75820 |
|---|---|
| 著者 | Minatogawa Y, Kawai C, Hatada S, Sato M. |
| タイトル | Liver specific kynurenine(alanine):glyoxylate aminotransferase was expressed in kidney cell line. |
| ジャーナル | Adv Exp Med Biol |
| Abstract |
Kynurenine (Alanine); glyoxylate aminotransferase (AGT) expression plasmid in the COS-7 was constructed. pGV-C was used as a expression vector which contains SV-40 promoter and enhancer. pGV-C and human AGT clone, H1-2, were digested by Hind III/Sma I separately. The AGT fragment was inserted into the digested pGV-C large fragment, The constructed plasmid was named as pGV-AGT. The constructed plasmid was transfected to COS-7 cultured cell by electroporation. The best electroporation condition was checked. |
| 巻・号 | 398 |
| ページ | 471-6 |
| 公開日 | 1996-1-1 |
| PMID | 8906307 |
| MeSH | Alanine Transaminase / biosynthesis Alanine Transaminase / metabolism* Animals COS Cells Humans Kidney Kinetics Liver / enzymology* Plasmids Recombinant Proteins / metabolism Substrate Specificity Time Factors Transaminases* Transfection |
| IF | 2.45 |
| リソース情報 | |
| ヒト・動物細胞 | COS-7(RCB0539) |