RRC ID 75821
Author Kita R, Nishida N, Fukuda Y, Azechi H, Matsuoka Y, Komeda T, Sando T, Nakao K, Ishizaki K.
Title Infrequent alterations of the p16INK4A gene in liver cancer.
Journal Int J Cancer
Abstract We examined the genomic status of the p16INK4A (inhibitor of cyclin-dependent kinase 4 A) and cyclin-dependent kinase 4 (CDK4) genes in 62 human hepatocellular carcinomas (HCCs), 5 cholangiocellular carcinomas and 6 cell lines derived from human liver cancers. Although no samples showed the homozygous deletion of the p16INK4A gene, we detected intragenic mutations of the p16INK4A gene in 3 HCCs and one HCC cell line, which led to an amino-acid substitution or a frameshift. In 2 HCC samples with mis-sense mutations of the p16INK4A gene, loss of heterozygosity on 9p22 was also detected, suggesting that the loss of function of p16 was induced during hepatocarcinogenesis. On the other hand, amplification or rearrangement of the CDK4 gene was not detected in any samples examined in this study. These results indicated that the mutations or deletions of the p16INK4A gene are not frequent, but may play a role in a sub-set of human HCC.
Volume 67(2)
Pages 176-80
Published 1996-7-17
DOI 10.1002/(SICI)1097-0215(19960717)67:2<176::AID-IJC4>3.0.CO;2-Q
PII 10.1002/(SICI)1097-0215(19960717)67:2<176::AID-IJC4>3.0.CO;2-Q
PMID 8760583
MeSH Base Sequence Carcinoma, Hepatocellular / genetics* Carrier Proteins / genetics* Cyclin-Dependent Kinase 4 Cyclin-Dependent Kinase Inhibitor p16 Cyclin-Dependent Kinases / genetics* Exons Gene Deletion Homozygote Humans Liver Neoplasms / genetics* Molecular Sequence Data Mutation* Polymerase Chain Reaction Proto-Oncogene Proteins* Tumor Cells, Cultured
IF 5.145
Human and Animal Cells Hep G2(RCB0459)