Reference - Detail
| RRC ID | 75878 |
|---|---|
| Author | Tsata V, Möllmert S, Schweitzer C, Kolb J, Möckel C, Böhm B, Rosso G, Lange C, Lesche M, Hammer J, Kesavan G, Beis D, Guck J, Brand M, Wehner D. |
| Title | A switch in pdgfrb+ cell-derived ECM composition prevents inhibitory scarring and promotes axon regeneration in the zebrafish spinal cord. |
| Journal | Dev Cell |
| Abstract |
In mammals, perivascular cell-derived scarring after spinal cord injury impedes axonal regrowth. In contrast, the extracellular matrix (ECM) in the spinal lesion site of zebrafish is permissive and required for axon regeneration. However, the cellular mechanisms underlying this interspecies difference have not been investigated. Here, we show that an injury to the zebrafish spinal cord triggers recruitment of pdgfrb+ myoseptal and perivascular cells in a PDGFR signaling-dependent manner. Interference with pdgfrb+ cell recruitment or depletion of pdgfrb+ cells inhibits axonal regrowth and recovery of locomotor function. Transcriptional profiling and functional experiments reveal that pdgfrb+ cells upregulate expression of axon growth-promoting ECM genes (cthrc1a and col12a1a/b) and concomitantly reduce synthesis of matrix molecules that are detrimental to regeneration (lum and mfap2). Our data demonstrate that a switch in ECM composition is critical for axon regeneration after spinal cord injury and identify the cellular source and components of the growth-promoting lesion ECM. |
| Volume | 56(4) |
| Pages | 509-524.e9 |
| Published | 2021-2-22 |
| DOI | 10.1016/j.devcel.2020.12.009 |
| PII | S1534-5807(20)30984-9 |
| PMID | 33412105 |
| MeSH | Animals Axons / metabolism* Cicatrix / pathology* Cicatrix / physiopathology Extracellular Matrix / metabolism* Models, Biological Nerve Regeneration* Receptor, Platelet-Derived Growth Factor beta / metabolism* Recovery of Function Signal Transduction Spinal Cord / pathology* Spinal Cord / physiopathology Spinal Cord Injuries / pathology Spinal Cord Injuries / physiopathology Zebrafish / physiology* Zebrafish Proteins / metabolism |
| IF | 10.092 |
| Resource | |
| Zebrafish | UAS:GFP |