論文 - 詳細
| RRC ID | 75895 |
|---|---|
| 著者 | Suzu S, Kimura F, Matsumoto H, Yamada M, Hashimoto K, Shimamura S, Motoyoshi K. |
| タイトル | Identification of binding domains for basic fibroblast growth factor in proteoglycan macrophage colony-stimulating factor. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
We recently demonstrated that proteoglycan macrophage colony-stimulating factor (PG-M-CSF) binds basic fibroblast growth factor (bFGF) and neutralizes the biological activity of bFGF. In this study, we identified the binding sites of PG-M-CSF for bFGF. We examined the binding of bFGF to overlapping 12-mer peptides with the sequence of the putative binding region. High affinity binding was detected at two peaks; one consisted of the three adjacent peptides, 212-223, 213-224 and 214-225 and the other, of the three adjacent peptides, 246-257, 247-258 and 248-259. The synthetic peptide (212VDPGSAKQRPPRST225) did not inhibit bFGF binding to another peptide (246PQPRPSVGAFNPGM259), and vice versa. However, both peptides inhibited the bFGF-induced but not platelet-derived growth factor-induced stimulation of DNA synthesis in murine Balb/c 3T3 cells. |
| 巻・号 | 230(2) |
| ページ | 392-7 |
| 公開日 | 1997-1-13 |
| DOI | 10.1006/bbrc.1996.5968 |
| PII | S0006-291X(96)95968-9 |
| PMID | 9016790 |
| MeSH | 3T3 Cells Amino Acid Sequence Animals Binding Sites CHO Cells Cell Division / drug effects Cricetinae DNA / biosynthesis Fibroblast Growth Factor 2 / chemistry* Fibroblast Growth Factor 2 / metabolism* Humans Kinetics Macrophage Colony-Stimulating Factor / chemistry* Macrophage Colony-Stimulating Factor / metabolism* Mice Molecular Sequence Data Peptide Fragments / chemical synthesis Peptide Fragments / chemistry Peptide Fragments / pharmacology* Proteoglycans / chemistry* Proteoglycans / metabolism* Recombinant Proteins / chemistry Recombinant Proteins / metabolism Transfection |
| IF | 2.985 |
| リソース情報 | |
| ヒト・動物細胞 | BALB/3T3 clone A31(RCB0005) |