| RRC ID |
75900
|
| 著者 |
Narasaki F, Oka M, Nakano R, Ikeda K, Fukuda M, Nakamura T, Soda H, Nakagawa M, Kuwano M, Kohno S.
|
| タイトル |
Human canalicular multispecific organic anion transporter (cMOAT) is expressed in human lung, gastric, and colorectal cancer cells.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
Human canalicular multispecific organic anion transporter (cMOAT), a glutathione conjugate membrane transporter, has been isolated from cisplatin-resistant cancer cells and is distributed mainly in normal liver. We analyzed the expression of human cMOAT in 14 lung, 11 gastric, and 9 colorectal non-drug-selected human cancer cells, two multidrug-resistant cells, and one cisplatin-resistant cells, using quantitative RT-PCR and newly developed anti-human cMOAT antibody. All cell lines analyzed here expressed human cMOAT at the level of mRNA and protein, and some of them expressed higher levels of human cMOAT than the cisplatin-resistant cells. The two multidrug-resistant cell lines co-expressed human cMOAT gene and both or either of MRP and MDR1 genes. Immunostaining showed that human cMOAT was predominantly localized to the cytoplasm of these single cells. Our results indicate that human cMOAT is expressed in various human cancer cells including drug-resistant cells.
|
| 巻・号 |
240(3)
|
| ページ |
606-11
|
| 公開日 |
1997-11-26
|
| DOI |
10.1006/bbrc.1997.7703
|
| PII |
S0006-291X(97)97703-2
|
| PMID |
9398612
|
| MeSH |
ATP Binding Cassette Transporter, Subfamily B, Member 1 / genetics
Anion Transport Proteins
Antineoplastic Agents / pharmacology
Carrier Proteins / biosynthesis
Carrier Proteins / genetics*
Cisplatin / pharmacology
Colorectal Neoplasms / genetics*
Colorectal Neoplasms / metabolism
Cytoplasm / metabolism
Drug Resistance, Multiple / genetics
Drug Resistance, Neoplasm / genetics
Gene Expression*
Genes, MDR / genetics
Humans
Immunoblotting
Immunohistochemistry
Lung Neoplasms / genetics*
Lung Neoplasms / metabolism
Polymerase Chain Reaction
RNA, Messenger / analysis
RNA, Messenger / genetics
Stomach Neoplasms / genetics*
Stomach Neoplasms / metabolism
Tumor Cells, Cultured
|
| IF |
2.985
|
| リソース情報 |
| ヒト・動物細胞 |
HGC-27(RCB0500) |
