RRC ID 75900
Author Narasaki F, Oka M, Nakano R, Ikeda K, Fukuda M, Nakamura T, Soda H, Nakagawa M, Kuwano M, Kohno S.
Title Human canalicular multispecific organic anion transporter (cMOAT) is expressed in human lung, gastric, and colorectal cancer cells.
Journal Biochem Biophys Res Commun
Abstract Human canalicular multispecific organic anion transporter (cMOAT), a glutathione conjugate membrane transporter, has been isolated from cisplatin-resistant cancer cells and is distributed mainly in normal liver. We analyzed the expression of human cMOAT in 14 lung, 11 gastric, and 9 colorectal non-drug-selected human cancer cells, two multidrug-resistant cells, and one cisplatin-resistant cells, using quantitative RT-PCR and newly developed anti-human cMOAT antibody. All cell lines analyzed here expressed human cMOAT at the level of mRNA and protein, and some of them expressed higher levels of human cMOAT than the cisplatin-resistant cells. The two multidrug-resistant cell lines co-expressed human cMOAT gene and both or either of MRP and MDR1 genes. Immunostaining showed that human cMOAT was predominantly localized to the cytoplasm of these single cells. Our results indicate that human cMOAT is expressed in various human cancer cells including drug-resistant cells.
Volume 240(3)
Pages 606-11
Published 1997-11-26
DOI 10.1006/bbrc.1997.7703
PII S0006-291X(97)97703-2
PMID 9398612
MeSH ATP Binding Cassette Transporter, Subfamily B, Member 1 / genetics Anion Transport Proteins Antineoplastic Agents / pharmacology Carrier Proteins / biosynthesis Carrier Proteins / genetics* Cisplatin / pharmacology Colorectal Neoplasms / genetics* Colorectal Neoplasms / metabolism Cytoplasm / metabolism Drug Resistance, Multiple / genetics Drug Resistance, Neoplasm / genetics Gene Expression* Genes, MDR / genetics Humans Immunoblotting Immunohistochemistry Lung Neoplasms / genetics* Lung Neoplasms / metabolism Polymerase Chain Reaction RNA, Messenger / analysis RNA, Messenger / genetics Stomach Neoplasms / genetics* Stomach Neoplasms / metabolism Tumor Cells, Cultured
IF 2.985
Human and Animal Cells HGC-27(RCB0500)