論文 - 詳細
| RRC ID | 75990 |
|---|---|
| 著者 | Knupp D, Jorgensen BG, Alshareef HZ, Bhat JM, Grubbs JJ, Miura P, van der Linden AM. |
| タイトル | Loss of circRNAs from the crh-1 gene extends the mean lifespan in Caenorhabditis elegans. |
| ジャーナル | Aging Cell |
| Abstract |
Accumulation of circular RNAs (circRNAs) during aging occurs on a genome-wide level for multiple organisms, but its significance is unknown. Generating circRNA loss-of-function mutants is difficult because the vast majority of these RNAs are comprised of exons shared with protein-coding mRNAs. In Caenorhabditis elegans, most circRNAs were previously found to accumulate during aging. Two of the most abundant, age-accumulating circRNAs are generated from exon 4 of the crh-1 gene (circ-crh-1). Here, we found that the biogenesis of circ-crh-1 was regulated by the double-stranded RNA-binding protein ADR-1. We identified Reverse Complementary Match (RCM) sequences in introns flanking circ-crh-1. Using CRISPR-Cas9, we deleted the downstream RCM and found that this completely eliminated expression of the circRNA without affecting linear mRNA expression from the crh-1 gene. Remarkably, worms lacking circ-crh-1 exhibited a significantly longer mean lifespan. Lifespan was partially restored to wild type by expression of circ-crh-1 in neural tissues. Widespread transcriptome alterations in circ-crh-1 mutants were identified using RNA-Seq. Moving forward, intronic RCM deletion using CRISPR should be a widely applicable method to identify lifespan-regulating circRNAs in C. elegans. |
| 巻・号 | 21(2) |
| ページ | e13560 |
| 公開日 | 2022-2-1 |
| DOI | 10.1111/acel.13560 |
| PMID | 35102684 |
| PMC | PMC8844124 |
| MeSH | Adenosine Deaminase* / genetics Adenosine Deaminase* / metabolism Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins* / genetics Caenorhabditis elegans Proteins* / metabolism Longevity / genetics RNA / metabolism RNA, Circular* / genetics Transcription Factors* / metabolism Transcriptome |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 10 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm668 |