論文 - 詳細
| RRC ID | 76022 |
|---|---|
| 著者 | Nguyen DAH, Phillips CM. |
| タイトル | Arginine methylation promotes siRNA-binding specificity for a spermatogenesis-specific isoform of the Argonaute protein CSR-1. |
| ジャーナル | Nat Commun |
| Abstract |
CSR-1 is an essential Argonaute protein that binds to a subclass of 22G-RNAs targeting most germline-expressed genes. Here we show that the two isoforms of CSR-1 have distinct expression patterns; CSR-1B is ubiquitously expressed throughout the germline and during all stages of development while CSR-1A expression is restricted to germ cells undergoing spermatogenesis. Furthermore, CSR-1A associates preferentially with 22G-RNAs mapping to spermatogenesis-specific genes whereas CSR-1B-bound small RNAs map predominantly to oogenesis-specific genes. Interestingly, the exon unique to CSR-1A contains multiple dimethylarginine modifications, which are necessary for the preferential binding of CSR-1A to spermatogenesis-specific 22G-RNAs. Thus, we have discovered a regulatory mechanism for C. elegans Argonaute proteins that allows for specificity of small RNA binding between similar Argonaute proteins with overlapping temporal and spatial localization. |
| 巻・号 | 12(1) |
| ページ | 4212 |
| 公開日 | 2021-7-9 |
| DOI | 10.1038/s41467-021-24526-6 |
| PII | 10.1038/s41467-021-24526-6 |
| PMID | 34244496 |
| PMC | PMC8270938 |
| MeSH | Animals Animals, Genetically Modified Arginine / metabolism Caenorhabditis elegans / genetics Caenorhabditis elegans / growth & development* Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism Exons / genetics Female Gene Expression Regulation, Developmental* Male Methylation Oogenesis / genetics Protein Binding / genetics Protein Isoforms / genetics Protein Isoforms / metabolism RNA Interference* RNA, Small Interfering / metabolism Sex Determination Processes / genetics Spermatogenesis / genetics* |
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| 最多言及媒体 | X(Twitter) |
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| 線虫 | tm1155 |