RRC ID 76022
Author Nguyen DAH, Phillips CM.
Title Arginine methylation promotes siRNA-binding specificity for a spermatogenesis-specific isoform of the Argonaute protein CSR-1.
Journal Nat Commun
Abstract CSR-1 is an essential Argonaute protein that binds to a subclass of 22G-RNAs targeting most germline-expressed genes. Here we show that the two isoforms of CSR-1 have distinct expression patterns; CSR-1B is ubiquitously expressed throughout the germline and during all stages of development while CSR-1A expression is restricted to germ cells undergoing spermatogenesis. Furthermore, CSR-1A associates preferentially with 22G-RNAs mapping to spermatogenesis-specific genes whereas CSR-1B-bound small RNAs map predominantly to oogenesis-specific genes. Interestingly, the exon unique to CSR-1A contains multiple dimethylarginine modifications, which are necessary for the preferential binding of CSR-1A to spermatogenesis-specific 22G-RNAs. Thus, we have discovered a regulatory mechanism for C. elegans Argonaute proteins that allows for specificity of small RNA binding between similar Argonaute proteins with overlapping temporal and spatial localization.
Volume 12(1)
Pages 4212
Published 2021-7-9
DOI 10.1038/s41467-021-24526-6
PII 10.1038/s41467-021-24526-6
PMID 34244496
PMC PMC8270938
MeSH Animals Animals, Genetically Modified Arginine / metabolism Caenorhabditis elegans / genetics Caenorhabditis elegans / growth & development* Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism Exons / genetics Female Gene Expression Regulation, Developmental* Male Methylation Oogenesis / genetics Protein Binding / genetics Protein Isoforms / genetics Protein Isoforms / metabolism RNA Interference* RNA, Small Interfering / metabolism Sex Determination Processes / genetics Spermatogenesis / genetics*
C.elegans tm1155