論文 - 詳細
| RRC ID | 76079 |
|---|---|
| 著者 | van der Woude M, Lans H. |
| タイトル | C. elegans survival assays to discern global and transcription-coupled nucleotide excision repair. |
| ジャーナル | STAR Protoc |
| Abstract |
Global genome nucleotide excision repair (GG-NER) and transcription-coupled nucleotide excision repair (TC-NER) protect cells against a variety of helix-distorting DNA lesions. In C. elegans, GG-NER primarily acts in proliferative germ cells and embryos, while TC-NER acts in post-mitotic somatic cells to maintain transcription. We leverage this difference to distinguish whether proteins function in GG-NER and/or TC-NER by straightforward UV survival assays. Here, we detail a protocol for these assays, using GG-NER factor xpc-1 and TC-NER factor csb-1 as examples. For complete details on the use and execution of this protocol, please refer to Sabatella et al. (2021). |
| 巻・号 | 2(2) |
| ページ | 100586 |
| 公開日 | 2021-6-18 |
| DOI | 10.1016/j.xpro.2021.100586 |
| PII | S2666-1667(21)00293-8 |
| PMID | 34151304 |
| PMC | PMC8192855 |
| MeSH | Animals Caenorhabditis elegans / physiology* DNA Repair* Transcription, Genetic* |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm3886 |