RRC ID 76079
Author van der Woude M, Lans H.
Title C. elegans survival assays to discern global and transcription-coupled nucleotide excision repair.
Journal STAR Protoc
Abstract Global genome nucleotide excision repair (GG-NER) and transcription-coupled nucleotide excision repair (TC-NER) protect cells against a variety of helix-distorting DNA lesions. In C. elegans, GG-NER primarily acts in proliferative germ cells and embryos, while TC-NER acts in post-mitotic somatic cells to maintain transcription. We leverage this difference to distinguish whether proteins function in GG-NER and/or TC-NER by straightforward UV survival assays. Here, we detail a protocol for these assays, using GG-NER factor xpc-1 and TC-NER factor csb-1 as examples. For complete details on the use and execution of this protocol, please refer to Sabatella et al. (2021).
Volume 2(2)
Pages 100586
Published 2021-6-18
DOI 10.1016/j.xpro.2021.100586
PII S2666-1667(21)00293-8
PMID 34151304
PMC PMC8192855
MeSH Animals Caenorhabditis elegans / physiology* DNA Repair* Transcription, Genetic*
C.elegans tm3886