RRC ID 76127
Author Brenner JL, Jyo EM, Mohammad A, Fox P, Jones V, Mardis E, Schedl T, Maine EM.
Title TRIM-NHL protein, NHL-2, modulates cell fate choices in the C. elegans germ line.
Journal Dev Biol
Abstract Many tissues contain multipotent stem cells that are critical for maintaining tissue function. In Caenorhabditis elegans, germline stem cells allow gamete production to continue in adulthood. In the gonad, GLP-1/Notch signaling from the distal tip cell niche to neighboring germ cells activates a complex regulatory network to maintain a stem cell population. GLP-1/Notch signaling positively regulates production of LST-1 and SYGL-1 proteins that, in turn, interact with a set of PUF/FBF proteins to positively regulate the stem cell fate. We previously described sog (suppressor of glp-1 loss of function) and teg (tumorous enhancer of glp-1 gain of function) genes that limit the stem cell fate and/or promote the meiotic fate. Here, we show that sog-10 is allelic to nhl-2. NHL-2 is a member of the conserved TRIM-NHL protein family whose members can bind RNA and ubiquitinate protein substrates. We show that NHL-2 acts, at least in part, by inhibiting the expression of PUF-3 and PUF-11 translational repressor proteins that promote the stem cell fate. Two other negative regulators of stem cell fate, CGH-1 (conserved germline helicase) and ALG-5 (Argonaute protein), may work with NHL-2 to modulate the stem cell population. In addition, NHL-2 activity promotes the male germ cell fate in XX animals.
Volume 491
Pages 43-55
Published 2022-11-1
DOI 10.1016/j.ydbio.2022.08.010
PII S0012-1606(22)00162-2
PMID 36063869
PMC PMC9922029
MeSH Animals Argonaute Proteins / metabolism Caenorhabditis elegans* / genetics Caenorhabditis elegans* / metabolism Caenorhabditis elegans Proteins* / genetics Caenorhabditis elegans Proteins* / metabolism Carrier Proteins / metabolism Germ Cells / metabolism Glucagon-Like Peptide 1 / genetics Glucagon-Like Peptide 1 / metabolism Male RNA / metabolism RNA Nucleotidyltransferases / metabolism RNA-Binding Proteins / genetics RNA-Binding Proteins / metabolism Receptors, Notch / metabolism Repressor Proteins / metabolism Transcription Factors / metabolism Tripartite Motif Proteins / metabolism
Resource
C.elegans tm1163