Reference - Detail
| RRC ID | 76253 |
|---|---|
| Author | Odiba AS, Ezechukwu CS, Liao G, Li S, Chen Z, Liu X, Fang W, Jin C, Wang B. |
| Title | Loss of NSE-4 Perturbs Genome Stability and DNA Repair in Caenorhabditis elegans. |
| Journal | Int J Mol Sci |
| Abstract |
The Structural Maintenance of Chromosomes (SMC) complex plays an important role in maintaining chromosome integrity, in which the SMC5/6 complex occupies a central position by facilitating mitotic and meiotic processes as well as DNA repair. NSE-4 Kleisin is critical for both the organization and function of the SMC5/6 complex, bridging NSE1 and NSE3 (MAGE related) with the head domains of the SMC5 and SMC6 proteins. Despite the conservation in protein sequence, no functional relevance of the NSE-4 homologous protein (NSE-4) in Caenorhabditis elegans has been reported. Here, we demonstrated the essential role of C. elegans NSE-4 in genome maintenance and DNA repair. Our results showed that NSE-4 is essential for the maintenance of chromosomal structure and repair of a range of chemically induced DNA damage. Furthermore, NSE-4 is involved in inter-sister repair during meiosis. NSE-4 localizes on the chromosome and is indispensable for the localization of NSE-1. Collectively, our data from this study provide further insight into the evolutionary conservation and diversification of NSE-4 function in the SMC-5/6 complex. |
| Volume | 23(13) |
| Published | 2022-6-29 |
| DOI | 10.3390/ijms23137202 |
| PII | ijms23137202 |
| PMID | 35806213 |
| PMC | PMC9266361 |
| MeSH | Animals Caenorhabditis elegans* / genetics Caenorhabditis elegans* / metabolism Cell Cycle Proteins* / metabolism DNA Repair Genomic Instability Meiosis |
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 4 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| C.elegans | tm7141 tm7158 tm1145 tm1937 |