| RRC ID |
76253
|
| 著者 |
Odiba AS, Ezechukwu CS, Liao G, Li S, Chen Z, Liu X, Fang W, Jin C, Wang B.
|
| タイトル |
Loss of NSE-4 Perturbs Genome Stability and DNA Repair in Caenorhabditis elegans.
|
| ジャーナル |
Int J Mol Sci
|
| Abstract |
The Structural Maintenance of Chromosomes (SMC) complex plays an important role in maintaining chromosome integrity, in which the SMC5/6 complex occupies a central position by facilitating mitotic and meiotic processes as well as DNA repair. NSE-4 Kleisin is critical for both the organization and function of the SMC5/6 complex, bridging NSE1 and NSE3 (MAGE related) with the head domains of the SMC5 and SMC6 proteins. Despite the conservation in protein sequence, no functional relevance of the NSE-4 homologous protein (NSE-4) in Caenorhabditis elegans has been reported. Here, we demonstrated the essential role of C. elegans NSE-4 in genome maintenance and DNA repair. Our results showed that NSE-4 is essential for the maintenance of chromosomal structure and repair of a range of chemically induced DNA damage. Furthermore, NSE-4 is involved in inter-sister repair during meiosis. NSE-4 localizes on the chromosome and is indispensable for the localization of NSE-1. Collectively, our data from this study provide further insight into the evolutionary conservation and diversification of NSE-4 function in the SMC-5/6 complex.
|
| 巻・号 |
23(13)
|
| 公開日 |
2022-6-29
|
| DOI |
10.3390/ijms23137202
|
| PII |
ijms23137202
|
| PMID |
35806213
|
| PMC |
PMC9266361
|
| MeSH |
Animals
Caenorhabditis elegans* / genetics
Caenorhabditis elegans* / metabolism
Cell Cycle Proteins* / metabolism
DNA Repair
Genomic Instability
Meiosis
|
| リソース情報 |
| 線虫 |
tm7141
tm7158
tm1145
tm1937 |
