論文 - 詳細
| RRC ID | 76283 |
|---|---|
| 著者 | Rourke C, Jaramillo-Lambert A. |
| タイトル | TOP-2 is differentially required for the proper maintenance of the cohesin subunit REC-8 on meiotic chromosomes in Caenorhabditis elegans spermatogenesis and oogenesis. |
| ジャーナル | Genetics |
| Abstract |
During meiotic prophase I, accurate segregation of homologous chromosomes requires the establishment of chromosomes with a meiosis-specific architecture. The sister chromatid cohesin complex and the enzyme Topoisomerase II (TOP-2) are important components of meiotic chromosome architecture, but the relationship of these proteins in the context of meiotic chromosome segregation is poorly defined. Here, we analyzed the role of TOP-2 in the timely release of the sister chromatid cohesin subunit REC-8 during spermatogenesis and oogenesis of Caenorhabditis elegans. We show that there is a different requirement for TOP-2 in meiosis of spermatogenesis and oogenesis. The loss-of-function mutation top-2(it7) results in premature REC-8 removal in spermatogenesis, but not oogenesis. This correlates with a failure to maintain the HORMA-domain proteins HTP-1 and HTP-2 (HTP-1/2) on chromosome axes at diakinesis and mislocalization of the downstream components that control REC-8 release including Aurora B kinase. In oogenesis, top-2(it7) causes a delay in the localization of Aurora B to oocyte chromosomes but can be rescued through premature activation of the maturation promoting factor via knockdown of the inhibitor kinase WEE-1.3. The delay in Aurora B localization is associated with an increase in the length of diakinesis bivalents and wee-1.3 RNAi mediated rescue of Aurora B localization in top-2(it7) is associated with a decrease in diakinesis bivalent length. Our results imply that the sex-specific effects of TOP-2 on REC-8 release are due to differences in the temporal regulation of meiosis and chromosome structure in late prophase I in spermatogenesis and oogenesis. |
| 巻・号 | 222(2) |
| 公開日 | 2022-9-30 |
| DOI | 10.1093/genetics/iyac120 |
| PII | 6661349 |
| PMID | 35951744 |
| PMC | PMC9526062 |
| MeSH | Animals Aurora Kinase B / metabolism Caenorhabditis elegans* / genetics Caenorhabditis elegans* / metabolism Caenorhabditis elegans Proteins* / metabolism Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism Chromatids / metabolism Chromosomal Proteins, Non-Histone Chromosome Segregation Chromosomes Cohesins DNA Topoisomerases, Type II / genetics DNA Topoisomerases, Type II / metabolism Female Male Maturation-Promoting Factor / genetics Maturation-Promoting Factor / metabolism Meiosis Protein Serine-Threonine Kinases Protein-Tyrosine Kinases / genetics Spermatogenesis / genetics |
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