論文 - 詳細
| RRC ID | 76313 |
|---|---|
| 著者 | Turan MG, Kantarci H, Temtek SD, Cakici O, Cevik S, Kaplan OI. |
| タイトル | Protocol for determining the average speed and frequency of kinesin and dynein-driven intraflagellar transport (IFT) in C. elegans. |
| ジャーナル | STAR Protoc |
| Abstract |
Here, we present a protocol to image a fluorescent-labeled intraflagellar transport (IFT) component in Caenorhabditis elegans with fluorescence microscopy, including steps of sample preparations, in vivo live-cell imaging, and post-microscopy analysis with kymographs. This protocol breaks down all processes into three categories: (1) pre-imaging preparations, (2) preparations for the time of image acquisition, and (3) post-imaging analyses. The protocol can be applied to determine the speed and frequency of moving particles. For complete details on the use and execution of this protocol, please refer to Cevik et al. (2021). |
| 巻・号 | 3(3) |
| ページ | 101498 |
| 公開日 | 2022-9-16 |
| DOI | 10.1016/j.xpro.2022.101498 |
| PII | S2666-1667(22)00378-1 |
| PMID | 35776634 |
| PMC | PMC9250072 |
| MeSH | Animals Caenorhabditis elegans* / metabolism Caenorhabditis elegans Proteins* / genetics Cilia / metabolism Dyneins / genetics Flagella / metabolism Kinesins Mutation |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm10423 |