RRC ID 76376
Author Ogasawara T, Watanabe J, Adachi R, Ono Y, Kamimura Y, Muramoto T.
Title CRISPR/Cas9-based genome-wide screening of Dictyostelium.
Journal Sci Rep
Abstract Genome-wide screening is powerful method used to identify genes and pathways associated with a phenotype of interest. The simple eukaryote Dictyostelium discoideum has a unique life cycle and is often used as a crucial research model for a wide range of biological processes and rare metabolites. To address the inadequacies of conventional genetic screening approaches, we developed a highly efficient CRISPR/Cas9-based genome-wide screening system for Dictyostelium. A genome-wide library of 27,405 gRNAs and a kinase library of 4,582 gRNAs were compiled and mutant pools were generated. The resulting mutants were screened for defects in cell growth and more than 10 candidate genes were identified. Six of these were validated and five recreated mutants presented with growth abnormalities. Finally, the genes implicated in developmental defects were screened to identify the unknown genes associated with a phenotype of interest. These findings demonstrate the potential of the CRISPR/Cas9 system as an efficient genome-wide screening method.
Volume 12(1)
Pages 11215
Published 2022-7-2
DOI 10.1038/s41598-022-15500-3
PII 10.1038/s41598-022-15500-3
PMID 35780186
PMC PMC9250498
MeSH CRISPR-Cas Systems / genetics Dictyostelium* / genetics Gene Library Genome RNA, Guide, Kinetoplastida / genetics
IF 3.998
Cellular slime molds G90641 G90426