論文 - 詳細
| RRC ID | 76379 |
|---|---|
| 著者 | Günther M, Reimer C, Herbst R, Kufs JE, Rautschek J, Ueberschaar N, Zhang S, Peschel G, Reimer L, Regestein L, Valiante V, Hillmann F, Stallforth P. |
| タイトル | Yellow polyketide pigment suppresses premature hatching in social amoeba. |
| ジャーナル | Proc Natl Acad Sci U S A |
| Abstract |
Low-molecular-weight natural products from microbes are indispensable in the development of potent drugs. However, their biological roles within an ecological context often remain elusive. Here, we shed light on natural products from eukaryotic microorganisms that have the ability to transition from single cells to multicellular organisms: the social amoebae. These eukaryotes harbor a large number of polyketide biosynthetic genes in their genomes, yet virtually none of the corresponding products can be isolated or characterized. Using complementary molecular biology approaches, including CRISPR-Cas9, we generated polyketide synthase (pks5) inactivation and overproduction strains of the social amoeba Dictyostelium discoideum. Differential, untargeted metabolomics of wild-type versus mutant fruiting bodies allowed us to pinpoint candidate metabolites derived from the amoebal PKS5. Extrachromosomal expression of the respective gene led to the identification of a yellow polyunsaturated fatty acid. Analysis of the temporospatial production pattern of this compound in conjunction with detailed bioactivity studies revealed the polyketide to be a spore germination suppressor. |
| 巻・号 | 119(43) |
| ページ | e2116122119 |
| 公開日 | 2022-10-25 |
| DOI | 10.1073/pnas.2116122119 |
| PMID | 36252029 |
| PMC | PMC9618038 |
| MeSH | Amoeba* / genetics Biological Products* / metabolism Dictyostelium* / physiology Polyketide Synthases / genetics Polyketide Synthases / metabolism Polyketides* / metabolism |
| IF | 9.412 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 21 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 細胞性粘菌 | G90426 |