| RRC ID |
76390
|
| 著者 |
Suzuki F, Miyamoto S, Takita M, Oshita M, Watanabe Y, Kakizuka A, Narumiya S, Taniguchi T, Muramatsu I.
|
| タイトル |
Cloning, functional expression and tissue distribution of rabbit alpha 1d-adrenoceptor.
|
| ジャーナル |
Biochim Biophys Acta
|
| Abstract |
We have cloned a cDNA encoding rabbit alpha 1d-adrenoceptor from the rabbit liver cDNA library. The deduced amino-acid sequence of this clone encodes a protein of 576 amino acids that shows strong sequence homology to previously cloned human, rat and mouse alpha 1d-adrenoceptors. The pharmacological radioligand binding properties of this clone expressed in COS-7 cells were similar to those of rat alpha 1d-adrenoceptors. Competitive RT/PCR assays revealed wide tissue distribution of the alpha 1d-adrenoceptor mRNA in rabbit, especially abundant in vas deferens, aorta, prostate and cerebral cortex.
|
| 巻・号 |
1323(1)
|
| ページ |
6-11
|
| 公開日 |
1997-1-14
|
| DOI |
10.1016/s0005-2736(96)00229-5
|
| PII |
S0005-2736(96)00229-5
|
| PMID |
9030207
|
| MeSH |
Amino Acid Sequence
Animals
Base Sequence
COS Cells
Cloning, Molecular
DNA Primers / genetics
DNA, Complementary / genetics
Gene Expression
Humans
Male
Mice
Molecular Sequence Data
Polymerase Chain Reaction
RNA, Messenger / genetics
RNA, Messenger / metabolism
Rabbits
Rats
Receptors, Adrenergic, alpha-1 / genetics*
Receptors, Adrenergic, alpha-1 / metabolism*
Sequence Homology, Amino Acid
Tissue Distribution
|
| IF |
3.411
|
| リソース情報 |
| ヒト・動物細胞 |
COS-7(RCB0539) |
