論文 - 詳細
| RRC ID | 76423 |
|---|---|
| 著者 | Morishita K, Kondo J, Sakon D, Hayashibara A, Tamura I, Shimizu K, Takamatsu S, Murata K, Kamada Y, Miyoshi E. |
| タイトル | Prohaptoglobin is a possible prognostic biomarker for colorectal cancer. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
BACKGROUND AND AIMS:Fucosylated haptoglobin is a novel glycan biomarker for colorectal and other cancers, while the significance of its precursor, prohaptoglobin (proHp), remains to be elucidated. In this study, we investigated whether proHp can be a colorectal cancer (CRC) biomarker and the biological functions of proHp in CRC using 10-7G, a monoclonal antibody recently developed in our laboratory. MATERIALS AND METHODS:Serum proHp level in 74 patients with CRC was semi-quantified by western blotting, and 5-year recurrence-free survival and overall survival were analyzed for groups stratified by proHp status (high vs. low). We also performed immunohistochemical analyses of 17 CRC tissue sections using 10-7G mAb. The biological functions of proHp were evaluated by overexpressing proHp in CRC cell lines. RESULTS:Serum proHp correlated with the clinical stage and poorer prognosis of CRC. In the primary CRC sections, immune cells were stained positive for 10-7G in ∼50% of the cases. Overexpression of proHp in HCT116 human CRC cells induced epithelial-mesenchymal transition-like changes and promoted cell migration in CRC cells. CONCLUSION:We provide evidence for the first time that proHp has potential as a prognostic biomarker for CRC and demonstrated specific biological activities of proHp. |
| 巻・号 | 672 |
| ページ | 72-80 |
| 公開日 | 2023-6-9 |
| DOI | 10.1016/j.bbrc.2023.06.017 |
| PII | S0006-291X(23)00760-X |
| PMID | 37343317 |
| MeSH | Cell Line, Tumor Cell Movement Cell Proliferation Colorectal Neoplasms* / metabolism Epithelial-Mesenchymal Transition HCT116 Cells Haptoglobins* / metabolism Humans Prognosis |
| IF | 2.985 |
| リソース情報 | |
| ヒト・動物細胞 | 293T(RCB2202) CACO-2(RCB0988) HCT116(RCB2979) LoVo(RCB1639) |