論文 - 詳細
| RRC ID | 76484 |
|---|---|
| 著者 | Kaneko H, Kaitsuka T, Tomizawa K. |
| タイトル | Artificial induction of circadian rhythm by combining exogenous BMAL1 expression and polycomb repressive complex 2 inhibition in human induced pluripotent stem cells. |
| ジャーナル | Cell Mol Life Sci |
| Abstract |
Understanding the physiology of human-induced pluripotent stem cells (iPSCs) is necessary for directed differentiation, mimicking embryonic development, and regenerative medicine applications. Pluripotent stem cells (PSCs) exhibit unique abilities such as self-renewal and pluripotency, but they lack some functions that are associated with normal somatic cells. One such function is the circadian oscillation of clock genes; however, whether or not PSCs demonstrate this capability remains unclear. In this study, the reason why circadian rhythm does not oscillate in human iPSCs was examined. This phenomenon may be due to the transcriptional repression of clock genes resulting from the hypermethylation of histone H3 at lysine 27 (H3K27), or it may be due to the low levels of brain and muscle ARNT-like 1 (BMAL1) protein. Therefore, BMAL1-overexpressing cells were generated and pre-treated with GSK126, an inhibitor of enhancer of zest homologue 2 (EZH2), which is a methyltransferase of H3K27 and a component of polycomb repressive complex 2. Consequently, a significant circadian rhythm following endogenous BMAL1, period 2 (PER2), and other clock gene expression was induced by these two factors, suggesting a candidate mechanism for the lack of rhythmicity of clock gene expression in iPSCs. |
| 巻・号 | 80(8) |
| ページ | 200 |
| 公開日 | 2023-7-8 |
| DOI | 10.1007/s00018-023-04847-z |
| PII | 10.1007/s00018-023-04847-z |
| PMID | 37421441 |
| MeSH | ARNTL Transcription Factors* / genetics ARNTL Transcription Factors* / metabolism Brain / metabolism CLOCK Proteins / genetics CLOCK Proteins / metabolism Circadian Clocks* / genetics Circadian Rhythm / physiology Humans Induced Pluripotent Stem Cells* / metabolism Muscles / metabolism Polycomb Repressive Complex 2* / metabolism |
| IF | 6.496 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | News |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 201B7(HPS0063) 1383D6(HPS1006) 1231A3(HPS0381) 585A1(HPS0354) |