論文 - 詳細
| RRC ID | 76491 |
|---|---|
| 著者 | Collins RT, Coxam B, Fechner I, Unterweger I, Szymborska A, Meier K, Gerhardt H. |
| タイトル | Intron with transgenic marker (InTraM) facilitates high-throughput screening of endogenous gene reporter lines. |
| ジャーナル | Genesis |
| Abstract |
The generation and maintenance of genome edited zebrafish lines is typically labor intensive due to the lack of an easy visual read-out for the modification. To facilitate this process, we have developed a novel method that relies on the inclusion of an artificial intron with a transgenic marker (InTraM) within the knock-in sequence of interest, which upon splicing produces a transcript with a precise and seamless modification. We have demonstrated this technology by replacing the stop codon of the zebrafish fli1a gene with a transcriptional activator KALTA4, using an InTraM that enables red fluorescent protein expression in the heart. |
| 巻・号 | 58(10-11) |
| ページ | e23391 |
| 公開日 | 2020-11-1 |
| DOI | 10.1002/dvg.23391 |
| PMID | 32783355 |
| MeSH | Animals CRISPR-Cas Systems Gene Editing / methods* Gene Knock-In Techniques / methods* Genes, Reporter* High-Throughput Screening Assays / methods* Transcription Factors / genetics Transgenes Zebrafish Zebrafish Proteins / genetics |
| IF | 1.76 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ゼブラフィッシュ | UAS:GFP |