論文 - 詳細
| RRC ID | 76638 |
|---|---|
| 著者 | Ho H, Kejzar N, Sasaguri H, Saito T, Saido TC, De Strooper B, Bauza M, Krupic J. |
| タイトル | A fully automated home cage for long-term continuous phenotyping of mouse cognition and behavior. |
| ジャーナル | Cell Rep Methods |
| Abstract |
Automated home-cage monitoring systems present a valuable tool for comprehensive phenotyping of natural behaviors. However, current systems often involve complex training routines, water or food restriction, and probe a limited range of behaviors. Here, we present a fully automated home-cage monitoring system for cognitive and behavioral phenotyping in mice. The system incorporates T-maze alternation, novel object recognition, and object-in-place recognition tests combined with monitoring of locomotion, drinking, and quiescence patterns, all carried out over long periods. Mice learn the tasks rapidly without any need for water or food restrictions. Behavioral characterization employs a deep convolutional neural network image analysis. We show that combined statistical properties of multiple behaviors can be used to discriminate between mice with hippocampal, medial entorhinal, and sham lesions and predict the genotype of an Alzheimer's disease mouse model with high accuracy. This technology may enable large-scale behavioral screening for genes and neural circuits underlying spatial memory and other cognitive processes. |
| 巻・号 | 3(7) |
| ページ | 100532 |
| 公開日 | 2023-7-24 |
| DOI | 10.1016/j.crmeth.2023.100532 |
| PII | S2667-2375(23)00168-6 |
| PMID | 37533650 |
| PMC | PMC10391580 |
| MeSH | Alzheimer Disease* / genetics Animals Behavior, Animal Cognition* Computers Hippocampus Mice |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 実験動物マウス | RBRC06344 |