| RRC ID |
76650
|
| 著者 |
Gujar MR, Gao Y, Teng X, Ding WY, Lin J, Tan YS, Chew LY, Toyama Y, Wang H.
|
| タイトル |
Patronin/CAMSAP promotes reactivation and regeneration of Drosophila quiescent neural stem cells.
|
| ジャーナル |
EMBO Rep
|
| Abstract |
The ability of stem cells to switch between quiescent and proliferative states is crucial for maintaining tissue homeostasis and regeneration. Drosophila quiescent neural stem cells (qNSCs) extend a primary protrusion that is enriched in acentrosomal microtubules and can be regenerated upon injury. Arf1 promotes microtubule growth, reactivation (exit from quiescence), and regeneration of qNSC protrusions upon injury. However, how Arf1 is regulated in qNSCs remains elusive. Here, we show that the microtubule minus-end binding protein Patronin/CAMSAP promotes acentrosomal microtubule growth and quiescent NSC reactivation. Patronin is important for the localization of Arf1 at Golgi and physically associates with Arf1, preferentially with its GDP-bound form. Patronin is also required for the regeneration of qNSC protrusion, likely via the regulation of microtubule growth. Finally, Patronin functions upstream of Arf1 and its effector Msps/XMAP215 to target the cell adhesion molecule E-cadherin to NSC-neuropil contact sites during NSC reactivation. Our findings reveal a novel link between Patronin/CAMSAP and Arf1 in the regulation of microtubule growth and NSC reactivation. A similar mechanism might apply to various microtubule-dependent systems in mammals.
|
| ページ |
e56624
|
| 公開日 |
2023-7-13
|
| DOI |
10.15252/embr.202256624
|
| PMID |
37440685
|
| PMC |
PMC10481672
|
| MeSH |
Animals
Drosophila / metabolism
Drosophila Proteins* / metabolism
Mammals / metabolism
Microtubule-Associated Proteins / metabolism
Microtubules / metabolism
Neural Stem Cells* / metabolism
|
| IF |
7.497
|
| リソース情報 |
| ショウジョウバエ |
18462 Ra-1 |
