論文 - 詳細
| RRC ID | 76688 |
|---|---|
| 著者 | Iriki T, Iio H, Yasuda S, Masuta S, Kato M, Kosako H, Hirayama S, Endo A, Ohtake F, Kamiya M, Urano Y, Saeki Y, Hamazaki J, Murata S. |
| タイトル | Senescent cells form nuclear foci that contain the 26S proteasome. |
| ジャーナル | Cell Rep |
| Abstract |
The proteasome plays a central role in intracellular protein degradation. Age-dependent decline in proteasome activity is associated with cellular senescence and organismal aging; however, the mechanism by which the proteasome plays a role in senescent cells remains elusive. Here, we show that nuclear foci that contain the proteasome and exhibit liquid-like properties are formed in senescent cells. The formation of senescence-associated nuclear proteasome foci (SANPs) is dependent on ubiquitination and RAD23B, similar to previously known nuclear proteasome foci, but also requires proteasome activity. RAD23B knockdown suppresses SANP formation and increases mitochondrial activity, leading to reactive oxygen species production without affecting other senescence traits such as cell-cycle arrest and cell morphology. These findings suggest that SANPs are an important feature of senescent cells and uncover a mechanism by which the proteasome plays a role in senescent cells. |
| ページ | 112880 |
| 公開日 | 2023-8-1 |
| DOI | 10.1016/j.celrep.2023.112880 |
| PII | S2211-1247(23)00891-4 |
| PMID | 37541257 |
| MeSH | Cell Nucleus* / metabolism Cellular Senescence Proteasome Endopeptidase Complex* / metabolism Ubiquitination |
| IF | 8.109 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 25 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | WI-38(RCB0702) |
| 遺伝子材料 | CSII-EF-MCS (RDB04378) pCAG-HIVgp (RDB04394) pCMV-VSV-G-RSV-Rev (RDB04393) |