論文 - 詳細
| RRC ID | 76700 |
|---|---|
| 著者 | Burke R, McCabe A, Sonawane NR, Rathod MH, Whelan CV, McCabe PF, Kacprzyk J. |
| タイトル | Arabidopsis cell suspension culture and RNA sequencing reveal regulatory networks underlying plant-programmed cell death. |
| ジャーナル | Plant J |
| Abstract |
Programmed cell death (PCD) facilitates selective, genetically controlled elimination of redundant, damaged, or infected cells. In plants, PCD is often an essential component of normal development and can mediate responses to abiotic and biotic stress stimuli. However, studying the transcriptional regulation of PCD is hindered by difficulties in sampling small groups of dying cells that are often buried within the bulk of living plant tissue. We addressed this challenge by using RNA sequencing and Arabidopsis thaliana suspension cells, a model system that allows precise monitoring of PCD rates. The use of three PCD-inducing treatments (salicylic acid, heat, and critical dilution), in combination with three cell death modulators (3-methyladenine, lanthanum chloride, and conditioned medium), enabled isolation of candidate core- and stimuli-specific PCD genes, inference of underlying regulatory networks and identification of putative transcriptional regulators of PCD in plants. This analysis underscored a disturbance of the cell cycle and mitochondrial retrograde signaling, and repression of pro-survival stress responses, as key elements of the PCD-associated transcriptional signature. Further, phenotyping of Arabidopsis T-DNA insertion mutants in selected candidate genes validated the potential of generated resources to identify novel genes involved in plant PCD pathways and/or stress tolerance. |
| 公開日 | 2023-8-2 |
| DOI | 10.1111/tpj.16407 |
| PMID | 37531399 |
| MeSH | Apoptosis / genetics Arabidopsis* / metabolism Arabidopsis Proteins* / genetics Arabidopsis Proteins* / metabolism Cell Death / genetics Gene Expression Regulation, Plant / genetics Sequence Analysis, RNA |
| IF | 6.141 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 12 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | psx00920 psx02307 psx04031 |