Reference - Detail
| RRC ID | 76734 |
|---|---|
| Author | Khateb A, Gago S, Bromley M, Richardson M, Bowyer P. |
| Title | Aneuploidy Is Associated with Azole Resistance in Aspergillus fumigatus. |
| Journal | Antimicrob Agents Chemother |
| Abstract |
Azole resistance in Aspergillus fumigatus is on the rise. Nontarget-mediated mechanisms are a common cause of azole resistance in chronic pulmonary aspergillosis (CPA). Here, we investigate resistance mechanisms using whole-genome sequencing. Sixteen azole-resistant A. fumigatus isolates from CPA were sequenced to assess genome rearrangements. Seven out of 16 CPA isolates showed genomic duplications compared to zero out of 18 invasive isolates. Duplication of regions, including cyp51A, increased gene expression. Our results suggest aneuploidy as an azole resistance mechanism in CPA. |
| Volume | 67(4) |
| Pages | e0125322 |
| Published | 2023-4-18 |
| DOI | 10.1128/aac.01253-22 |
| PMID | 36975834 |
| PMC | PMC10112202 |
| MeSH | Aneuploidy Antifungal Agents / pharmacology Antifungal Agents / therapeutic use Aspergillosis* / drug therapy Aspergillus fumigatus / genetics Aspergillus fumigatus / metabolism Azoles / pharmacology Drug Resistance, Fungal / genetics Fungal Proteins / genetics Fungal Proteins / metabolism Humans Microbial Sensitivity Tests Pulmonary Aspergillosis* / drug therapy |
| IF | 4.904 |
| Resource | |
| Pathogenic eukaryotic microorganisms | Aspergillus fumigatus IFM 58401 |