論文 - 詳細
| RRC ID | 76856 |
|---|---|
| 著者 | Watanabe M, Ishidate M Jr, Nohmi T. |
| タイトル | Sensitive method for the detection of mutagenic nitroarenes and aromatic amines: new derivatives of Salmonella typhimurium tester strains possessing elevated O-acetyltransferase levels. |
| ジャーナル | Mutat Res |
| Abstract |
Acetyl-CoA: N-hydroxyarylamine O-acetyltransferase is an enzyme involved in the intracellular metabolic activation of arylhydroxylamines derived from mutagenic nitroarenes and aromatic amines. The acetyltransferase gene of Salmonella typhimurium TA1538 was cloned into pBR322 and the plasmids harboring the gene were introduced into TA98 and TA100. The resulting strains (YG1024 and YG1029) had about 100 times higher 2-hydroxyamino-6-methyldipyrido[1,2-a:3',2'-d]-imidazole (N-hydroxy-Glu-P-1) O-acetyltransferase activity than TA1538 containing pBR322, and were extremely sensitive to the mutagenic actions of 2-nitrofluorene, 1-nitropyrene, 1,8-dinitropyrene, 2-amino-6-methyldipyrido[1,2-a:3',2-d)-imidazole (Glu-P-1), 2-aminofluorene and 2-aminoanthracene. These results indicate that the new strains permit the efficient detection of the mutagenicity of environmental nitroarenes and aromatic amines. |
| 巻・号 | 234(5) |
| ページ | 337-48 |
| 公開日 | 1990-10-1 |
| DOI | 10.1016/0165-1161(90)90044-o |
| PII | 0165-1161(90)90044-O |
| PMID | 2215547 |
| MeSH | Acetyltransferases* Acyltransferases / genetics Acyltransferases / metabolism* Amines / pharmacology* Cloning, Molecular Gene Library Mutagenicity Tests / methods Nitro Compounds / pharmacology* Plasmids Polycyclic Compounds / pharmacology* Restriction Mapping Salmonella typhimurium / drug effects Salmonella typhimurium / enzymology* Salmonella typhimurium / genetics |
| IF | 2.463 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Policy sources |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | ME10029 ME10031 |