| RRC ID |
76886
|
| 著者 |
Liang H, Ma X, Zhang Y, Liu Y, Liu N, Zhang W, Chen J, Liu B, Du W, Liu X, Yu L.
|
| タイトル |
The formation of migrasomes is initiated by the assembly of sphingomyelin synthase 2 foci at the leading edge of migrating cells.
|
| ジャーナル |
Nat Cell Biol
|
| Abstract |
The migrasome is an organelle of migrating cells with diverse physiological functions. How migrasome formation is initiated is unknown. We found that sphingomyelin is enriched in migrasomes and identified sphingomyelin synthase 2 (SMS2) as an essential protein for migrasome biogenesis. SMS2 assembles into immobile foci that adhere on the basal membrane at the leading edge. When cells migrate away, the SMS2 foci 'move' out of cells and into retraction fibres, where they become migrasome formation sites and eventually grow into migrasomes. Mechanistically, SMS2 foci seed migrasomes by converting ceramide to sphingomyelin, which is essential for migrasome formation. Furthermore, CerS5, which is required for the synthesis of long-chain ceramide, and CERT, which transports ceramide from the endoplasmic reticulum to Golgi, are both required for migrasome formation. Our data reveal the essential role of ceramide and sphingomyelin in migrasome formation and suggest that SMS2 forms basal membrane-surface-connecting structures that pre-determine where migrasomes will grow.
|
| 巻・号 |
25(8)
|
| ページ |
1173-1184
|
| 公開日 |
2023-8-1
|
| DOI |
10.1038/s41556-023-01188-8
|
| PII |
10.1038/s41556-023-01188-8
|
| PMID |
37488437
|
| MeSH |
Ceramides / metabolism
Endoplasmic Reticulum / metabolism
Sphingomyelins* / metabolism
Transferases (Other Substituted Phosphate Groups)* / genetics
Transferases (Other Substituted Phosphate Groups)* / metabolism
|
| IF |
20.042
|
| リソース情報 |
| 遺伝子材料 |
pET28/His6-mCherry-NT-Lys (RDB13499)
pET28/His6-EGFP-NT-Lys (RDB13498) |
