Reference - Detail
| RRC ID | 77025 |
|---|---|
| Author | Zeng J, Tang SY, Toh LL, Wang S. |
| Title | Generation of "Off-the-Shelf" Natural Killer Cells from Peripheral Blood Cell-Derived Induced Pluripotent Stem Cells. |
| Journal | Stem Cell Reports |
| Abstract |
Current donor cell-dependent strategies can only produce limited "made-to-order" therapeutic natural killer (NK) cells for limited patients. To provide unlimited "off-the-shelf" NK cells that serve many recipients, we designed and demonstrated a holistic manufacturing scheme to mass-produce NK cells from induced pluripotent stem cells (iPSCs). Starting with a highly accessible human cell source, peripheral blood cells (PBCs), we derived a good manufacturing practice-compatible iPSC source, PBC-derived iPSCs (PBC-iPSCs) for this purpose. Through our original protocol that excludes CD34+ cell enrichment and spin embryoid body formation, high-purity functional and expandable NK cells were generated from PBC-iPSCs. Above all, most of these NK cells expressed no killer cell immunoglobulin-like receptors (KIRs), which renders them unrestricted by recipients' human leukocyte antigen genotypes. Hence, we have established a practical "from blood cell to stem cells and back with less (less KIRs)" strategy to generate abundant "universal" NK cells from PBC-iPSCs for a wide range of patients. |
| Volume | 9(6) |
| Pages | 1796-1812 |
| Published | 2017-12-12 |
| DOI | 10.1016/j.stemcr.2017.10.020 |
| PII | S2213-6711(17)30475-7 |
| PMID | 29173894 |
| PMC | PMC5785702 |
| MeSH | Cell Culture Techniques / methods* Cell Differentiation / genetics* Cell Proliferation / genetics Embryoid Bodies / cytology Gene Expression Regulation, Developmental / genetics Humans Induced Pluripotent Stem Cells / cytology* Induced Pluripotent Stem Cells / metabolism Killer Cells, Natural / cytology* Killer Cells, Natural / metabolism Peripheral Blood Stem Cells / cytology Peripheral Blood Stem Cells / metabolism Receptors, KIR / genetics |
| IF | 6.032 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 50 |
| Altmetric score changes over past 6months | 3.0 |
| Resource | |
| Human and Animal Cells | OP9/DLL1 |