| RRC ID |
77036
|
| Author |
Kita Y, Nishibe H, Wang Y, Hashikawa T, Kikuchi SS, U M, Yoshida AC, Yoshida C, Kawase T, Ishii S, Skibbe H, Shimogori T.
|
| Title |
Cellular-resolution gene expression profiling in the neonatal marmoset brain reveals dynamic species- and region-specific differences.
|
| Journal |
Proc Natl Acad Sci U S A
|
| Abstract |
Precise spatiotemporal control of gene expression in the developing brain is critical for neural circuit formation, and comprehensive expression mapping in the developing primate brain is crucial to understand brain function in health and disease. Here, we developed an unbiased, automated, large-scale, cellular-resolution in situ hybridization (ISH)-based gene expression profiling system (GePS) and companion analysis to reveal gene expression patterns in the neonatal New World marmoset cortex, thalamus, and striatum that are distinct from those in mice. Gene-ontology analysis of marmoset-specific genes revealed associations with catalytic activity in the visual cortex and neuropsychiatric disorders in the thalamus. Cortically expressed genes with clear area boundaries were used in a three-dimensional cortical surface mapping algorithm to delineate higher-order cortical areas not evident in two-dimensional ISH data. GePS provides a powerful platform to elucidate the molecular mechanisms underlying primate neurobiology and developmental psychiatric and neurological disorders.
|
| Volume |
118(18)
|
| Published |
2021-5-4
|
| DOI |
10.1073/pnas.2020125118
|
| PII |
2020125118
|
| PMID |
33903237
|
| PMC |
PMC8106353
|
| MeSH |
Animals
Animals, Newborn / genetics
Animals, Newborn / growth & development
Brain / growth & development
Brain / metabolism*
Callithrix / genetics*
Callithrix / growth & development
Corpus Striatum / growth & development
Corpus Striatum / metabolism
Gene Expression Regulation, Developmental / genetics
Humans
In Situ Hybridization
Mice
Species Specificity
Transcriptome / genetics*
Visual Cortex / growth & development
Visual Cortex / metabolism
|
| IF |
9.412
|
| Resource |
| DNA material |
MMTD00001 (MMTD00001) |
