論文 - 詳細
| RRC ID | 77051 |
|---|---|
| 著者 | Shiba H, Nishio M, Sawada M, Tamaki M, Michigami M, Nakai S, Nakase I, Fujii I, Matsumoto A, Kojima C. |
| タイトル | Carboxy-terminal dendrimers with phenylalanine for a pH-sensitive delivery system into immune cells including T cells. |
| ジャーナル | J Mater Chem B |
| Abstract |
Although T cells play important roles in various immune reactions, there are only a few reports on delivery systems into T cells. Our previous study showed that carboxy-terminal phenylalanine (Phe)-modified polyamidoamine (PAMAM) dendrimers have both temperature- and pH-sensitive properties, which are affected by the chemical structure. The self-assembled structures of Phe, observed in phenylketonuria, enhance the protein aggregation, the association with the cell membrane and the membrane permeability. In this study, we applied the Phe-modified dendrimers to a pH-sensitive drug delivery system into T cells. Dendrimers with different amino acids and acid anhydrides were synthesized, and their pH-responsive association with T cells and their subsets was investigated. The dendrimers modified with Phe and cyclohexanedicarboxylic acid (CHex) showed higher uptake into various cells, including Jurkat cells, CD3+ T cells, CD3 + CD4+ helper T cells and CD3 + CD8+ killer T cells. These dendrimers were internalized into T cells via endocytosis, and their cellular uptake was enhanced under weak acidic conditions (pH 6.5). Our results showed that Phe- and CHex-modified dendrimers have a delivery potential to T cells and their subsets, which may be useful for cancer immunotherapy. |
| 巻・号 | 10(14) |
| ページ | 2463-2470 |
| 公開日 | 2022-4-6 |
| DOI | 10.1039/d1tb01980e |
| PMID | 34935852 |
| MeSH | Cell Membrane Permeability Dendrimers* / chemistry Dendrimers* / pharmacology Drug Delivery Systems Humans Hydrogen-Ion Concentration Phenylalanine / chemistry Phenylalanine / pharmacology |
| IF | 5.344 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | News |
| 各媒体での言及数の合計 | 8 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |