RRC ID 77100
著者 Ito S, Ueda T, Yokoyama A, Fujihara A, Hongo F, Ukimura O.
タイトル PCA3 controls chromatin organization and p53 signal activation by regulating LAP2α-lamin A complexes.
ジャーナル Cancer Gene Ther
Abstract Prostate cancer antigen 3 (PCA3) is a prostate cancer-specific long noncoding RNA (lncRNA). Here, we report that lncRNA PCA3 plays a role in prostate cancer progression that is mediated by nucleoplasmic lamins. PCA3 interacts with the C-terminal region of lamina-associated polypeptide (LAP) 2α. The C-terminal region of LAP2α includes tumor suppressor protein retinoblastoma (pRb)- and lamin-binding domains, and it is necessary for the regulation and stabilization of the nucleoplasmic pool of lamin A. PCA3 inhibits the interaction of LAP2α with lamin A through binding with the C-terminus of LAP2α. The level of nucleoplasmic lamin A/C is increased by knockdown of PCA3. Together, the level of LAP2α within the nucleus is increased by PCA3 knockdown. In PCA3 knockdown cells, the levels of HP1γ, trimethylation of Lys9 on histone H3 (H3K9me3), and trimethylation of Lys36 on histone H3 (H3K36me3) are upregulated. In contrast, trimethylation of Lys4 on histone H3 (H3K4me3) is downregulated. We further demonstrate that activation of the p53 signaling pathway and cell cycle arrest are promoted in the absence of PCA3. These findings support a unique mechanism in which prostate cancer-specific lncRNA controls chromatin organization via regulation of the nucleoplasmic pool of lamins. This proposed mechanism suggests that cancer progression may be mediated by nuclear lamins.
巻・号 29(3-4)
ページ 358-368
公開日 2022-3-1
DOI 10.1038/s41417-021-00314-8
PII 10.1038/s41417-021-00314-8
PMID 33758375
MeSH Antigens, Neoplasm Chromatin / genetics DNA-Binding Proteins Histones / genetics Humans Lamin Type A / genetics Lamin Type A / metabolism Male Membrane Proteins Prostatic Neoplasms* / genetics RNA, Long Noncoding* / genetics Tumor Suppressor Protein p53 / genetics
IF 4.534
リソース情報
ヒト・動物細胞 LNCap.FGC(RCB2144) DU145(RCB2143) PC-3(RCB2145)