RRC ID 77150
Author Mawardi H, Giro G, Kajiya M, Ohta K, Almazrooa S, Alshwaimi E, Woo SB, Nishimura I, Kawai T.
Title A role of oral bacteria in bisphosphonate-induced osteonecrosis of the jaw.
Journal J Dent Res
Abstract No consensus has yet been reached to associate oral bacteria conclusively with the etio-pathogenesis of bisphosphonate-induced osteonecrosis of the jaw (BONJ). Therefore, the present study examined the effects of oral bacteria on the development of BONJ-like lesions in a mouse model. In the pamidronate (Pam)-treated mice, but not control non-drug-treated mice, tooth extraction followed by oral infection with Fusobacterium nucleatum caused BONJ-like lesions and delayed epithelial healing, both of which were completely suppressed by a broad-spectrum antibiotic cocktail. Furthermore, in both in vitro and in vivo experiments, the combination of Pam and Fusobacterium nucleatum caused the death of gingival fibroblasts (GFs) and down-regulated their production of keratinocyte growth factor (KGF), which induces epithelial cell growth and migration. Therefore, in periodontal tissues pre-exposed to bisphosphonate, bacterial infection at tooth extraction sites caused diminished KGF expression in GFs, leading to a delay in the epithelial wound-healing process that was mitigated by antibiotics.
Volume 90(11)
Pages 1339-45
Published 2011-11-1
DOI 10.1177/0022034511420430
PII 0022034511420430
PMID 21921248
PMC PMC3188458
MeSH Animals Anti-Bacterial Agents / therapeutic use Apoptosis Bone Density Conservation Agents / adverse effects Cell Movement Cell Survival Cells, Cultured Diphosphonates / adverse effects Disease Models, Animal Epithelial Cells / metabolism Epithelial Cells / microbiology Female Fibroblast Growth Factor 7 / biosynthesis Fibroblasts / metabolism Fibroblasts / microbiology Fusobacterium nucleatum / pathogenicity* Gingiva / cytology Gingiva / microbiology Jaw Diseases / chemically induced Jaw Diseases / microbiology* Mice Osteonecrosis / chemically induced Osteonecrosis / microbiology* Pamidronate Surgical Wound Infection / drug therapy Surgical Wound Infection / microbiology* Tooth Extraction / adverse effects
IF 4.914
Human and Animal Cells GE1(RCB1709)