論文 - 詳細
| RRC ID | 77158 |
|---|---|
| 著者 | Hori T, Kondo T, Kanamori M, Tabuchi Y, Ogawa R, Zhao QL, Ahmed K, Yasuda T, Seki S, Suzuki K, Kimura T. |
| タイトル | Ionizing radiation enhances tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced apoptosis through up-regulations of death receptor 4 (DR4) and death receptor 5 (DR5) in human osteosarcoma cells. |
| ジャーナル | J Orthop Res |
| Abstract |
Despite improvements in chemotherapy and surgery in the treatment of osteosarcoma (OS), satisfactory results are still difficult to achieve. Novel therapeutic modalities need to be developed for osteosarcoma treatment. The combined effects of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and ionizing radiation (IR) on human OS cells were investigated. IR and TRAIL treatment synergistically decreased the cell viability and enhanced apoptosis in OS cell lines. IR pretreatment enhances TRAIL-induced Bid and caspase-3 activations. Decreases in the expression levels of the antiapoptotic proteins c-FLIP and XIAP also associated with apoptosis enhancement. Furthermore, IR pretreatment enhanced DR4 and DR5 expressions at the transcription stage. These results can become the basic lines of evidence for the future treatment of OS using TRAIL with IR. |
| 巻・号 | 28(6) |
| ページ | 739-45 |
| 公開日 | 2010-6-1 |
| DOI | 10.1002/jor.21056 |
| PMID | 20041491 |
| MeSH | Apoptosis / drug effects* Bone Neoplasms / metabolism Bone Neoplasms / pathology Bone Neoplasms / radiotherapy* Cell Line, Tumor Cell Survival / drug effects Cell Survival / radiation effects Gene Expression Regulation, Neoplastic / radiation effects* Humans Osteosarcoma / metabolism Osteosarcoma / pathology Osteosarcoma / radiotherapy* Promoter Regions, Genetic RNA, Messenger / analysis Receptors, TNF-Related Apoptosis-Inducing Ligand / genetics* Receptors, Tumor Necrosis Factor / genetics* TNF-Related Apoptosis-Inducing Ligand / pharmacology* Up-Regulation |
| IF | 2.728 |
| リソース情報 | |
| ヒト・動物細胞 | Saos-2(RCB0428) HOS(RCB0992) |