| RRC ID |
77236
|
| 著者 |
Kurata N, Shishido T, Muraoka M, Tanaka T, Ogino C, Fukuda H, Kondo A.
|
| タイトル |
Specific protein delivery to target cells by antibody-displaying bionanocapsules.
|
| ジャーナル |
J Biochem
|
| Abstract |
Bionanocapsules (BNCs) are nanoparticles with a high biocompatibility composed of the L protein of the hepatitis B virus surface antigen. BNC can deliver bioactive molecules to hepatocytes efficiently and specifically. However, delivery is limited to hepatocytes and incorporation of proteins into BNC is quite troublesome. Here, in order to alter the specificity of BNC and to achieve efficient protein delivery, we developed engineered BNC displaying the ZZ domain of protein A and incorporating enhanced green fluorescent protein (EGFP) inside the particles using an insect cell expression system. The ZZ domain displayed on the surface of BNC binds to anti-epidermal growth factor receptor (EGFR) antibodies, allowing specific delivery of EGFP to HeLa cells. The engineered BNCs are a promising and powerful tool for efficient and cell-specific protein delivery.
|
| 巻・号 |
144(6)
|
| ページ |
701-7
|
| 公開日 |
2008-12-1
|
| DOI |
10.1093/jb/mvn131
|
| PII |
mvn131
|
| PMID |
18838435
|
| MeSH |
Antibodies / chemistry
Antibodies / metabolism
Drug Carriers / chemistry*
Drug Delivery Systems / methods
ErbB Receptors / chemistry
ErbB Receptors / metabolism
Genetic Vectors / genetics
Genetic Vectors / metabolism
Green Fluorescent Proteins / chemistry
Green Fluorescent Proteins / metabolism
HeLa Cells
Humans
Nanoparticles / chemistry*
Protein Engineering / methods
Proteins / administration & dosage*
|
| IF |
2.476
|
| リソース情報 |
| ヒト・動物細胞 |
HeLa(RCB0007)
Hep G2 |
