論文 - 詳細
| RRC ID | 77236 |
|---|---|
| 著者 | Kurata N, Shishido T, Muraoka M, Tanaka T, Ogino C, Fukuda H, Kondo A. |
| タイトル | Specific protein delivery to target cells by antibody-displaying bionanocapsules. |
| ジャーナル | J Biochem |
| Abstract |
Bionanocapsules (BNCs) are nanoparticles with a high biocompatibility composed of the L protein of the hepatitis B virus surface antigen. BNC can deliver bioactive molecules to hepatocytes efficiently and specifically. However, delivery is limited to hepatocytes and incorporation of proteins into BNC is quite troublesome. Here, in order to alter the specificity of BNC and to achieve efficient protein delivery, we developed engineered BNC displaying the ZZ domain of protein A and incorporating enhanced green fluorescent protein (EGFP) inside the particles using an insect cell expression system. The ZZ domain displayed on the surface of BNC binds to anti-epidermal growth factor receptor (EGFR) antibodies, allowing specific delivery of EGFP to HeLa cells. The engineered BNCs are a promising and powerful tool for efficient and cell-specific protein delivery. |
| 巻・号 | 144(6) |
| ページ | 701-7 |
| 公開日 | 2008-12-1 |
| DOI | 10.1093/jb/mvn131 |
| PII | mvn131 |
| PMID | 18838435 |
| MeSH | Antibodies / chemistry Antibodies / metabolism Drug Carriers / chemistry* Drug Delivery Systems / methods ErbB Receptors / chemistry ErbB Receptors / metabolism Genetic Vectors / genetics Genetic Vectors / metabolism Green Fluorescent Proteins / chemistry Green Fluorescent Proteins / metabolism HeLa Cells Humans Nanoparticles / chemistry* Protein Engineering / methods Proteins / administration & dosage* |
| IF | 2.476 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa(RCB0007) Hep G2 |