Reference - Detail
| RRC ID | 77267 |
|---|---|
| Author | Baba K, Uemura K, Nakazato R, Ijaz F, Takahashi S, Ikegami K. |
| Title | Δ3-tubulin impairs mitotic spindle morphology and increases nuclear size in pancreatic cancer cells. |
| Journal | Med Mol Morphol |
| Abstract |
Cancer cell proliferation is affected by post-translational modifications of tubulin. Especially, overexpression or depletion of enzymes for modifications on the tubulin C-terminal region perturbs dynamic instability of the spindle body. Those modifications include processing of C-terminal amino acids of α-tubulin; detyrosination, and a removal of penultimate glutamic acid (Δ2). We previously found a further removal of the third last glutamic acid, which generates so-called Δ3-tubulin. The effects of Δ3-tubulin on spindle integrities and cell proliferation remain to be elucidated. In this study, we investigated the impacts of forced expression of Δ3-tubulin on the structure of spindle bodies and cell division in a pancreatic cancer cell line, PANC-1. Overexpression of HA-tagged Δ3-tubulin impaired the morphology and orientation of spindle bodies during cell division in PANC-1 cells. In particular, spindle bending was most significantly increased. Expression of EGFP-tagged Δ3-tubulin driven by the endogenous promoter of human TUBA1B also deformed and misoriented spindle bodies. Spindle bending and condensation defects were significantly observed by EGFP-Δ3-tubulin expression. Furthermore, EGFP-Δ3-tubulin expression increased the nuclear size in a dose-dependent manner of EGFP-Δ3-tubulin expression. The expression of EGFP-Δ3-tubulin tended to slow down cell proliferation. Taken together, our results demonstrate that Δ3-tubulin affects the spindle integrity and cell division. |
| Volume | 57(1) |
| Pages | 59-67 |
| Published | 2024-3-1 |
| DOI | 10.1007/s00795-023-00373-w |
| PII | 10.1007/s00795-023-00373-w |
| PMID | 37930423 |
| MeSH | Glutamates / metabolism Glutamates / pharmacology Humans Microtubules / metabolism Mitosis Pancreatic Neoplasms* / genetics Pancreatic Neoplasms* / metabolism Spindle Apparatus / genetics Spindle Apparatus / metabolism Tubulin* / genetics Tubulin* / metabolism |
| IF | 2.429 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 4 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | PANC-1(RCB2095) |