RRC ID 77313
Author Shiura H, Ikeda R, Lee J, Sato T, Ogonuki N, Hirose M, Ogura A, Ogawa T, Abe K.
Title Generation of a novel germline stem cell line expressing a germline-specific reporter in the mouse.
Journal Genesis
Abstract Germline stem (GS) cells are stem cell lines derived from postnatal male germline cells. Remarkably, GS cells can form functional spermatozoa when transplanted into infertile host mouse testes, indicating that GS cells have spermatogonial stem cell (SSC) activity. As GS cells are the only type with SSC activity, they are most suitable for in vitro studies on male germ cell differentiation. However, GS cells can deviate from the germ cell state to become other types of cells, depending on culture conditions. Therefore, it is desirable to have a monitor system to ensure that GS cells are kept at the germ cell state in culture. Here, we established GS cell lines from neonatal testes of transgenic mice that express the fluorescent protein, Venus, whose gene expression is driven by the promoter of Mvh (mouse Vasa homolog), a gene highly specific to mammalian germ cells. This novel cell line has genuine GS cell properties equivalent to existing GS lines, including the ability to generate viable offspring. This Mvh-Venus GS cell line, to our knowledge, is the first one expressing a germ cell-specific reporter. This valuable resource should provide new opportunities for studies on male germ cell differentiation.
Volume 51(7)
Pages 498-505
Published 2013-7-1
DOI 10.1002/dvg.22391
PMID 23529902
MeSH Animals Bacterial Proteins / genetics Bacterial Proteins / metabolism Cell Line* DEAD-box RNA Helicases / genetics* DEAD-box RNA Helicases / metabolism DNA Methylation Genes, Reporter* Germ Cells* / cytology Germ Cells* / metabolism Luminescent Proteins / genetics Luminescent Proteins / metabolism Male Mice Mice, Inbred C57BL Mice, Transgenic Phenotype Stem Cells* / cytology Stem Cells* / metabolism Stem Cells* / physiology Testis / cytology* Testis / metabolism Testis / physiology
IF 1.76
Human and Animal Cells GS-DG1(AES0002)