| RRC ID |
77416
|
| 著者 |
Saito K, Ishizu H, Komai M, Kotani H, Kawamura Y, Nishida KM, Siomi H, Siomi MC.
|
| タイトル |
Roles for the Yb body components Armitage and Yb in primary piRNA biogenesis in Drosophila.
|
| ジャーナル |
Genes Dev
|
| Abstract |
PIWI-interacting RNAs (piRNAs) protect genome integrity from transposons. In Drosophila ovarian somas, primary piRNAs are produced and loaded onto Piwi. Here, we describe roles for the cytoplasmic Yb body components Armitage and Yb in somatic primary piRNA biogenesis. Armitage binds to Piwi and is required for localizing Piwi into Yb bodies. Without Armitage or Yb, Piwi is freed from the piRNAs and does not enter the nucleus. Thus, piRNA loading is required for Piwi nuclear entry. We propose that a functional Piwi-piRNA complex is formed and inspected in Yb bodies before its nuclear entry to exert transposon silencing.
|
| 巻・号 |
24(22)
|
| ページ |
2493-8
|
| 公開日 |
2010-11-15
|
| DOI |
10.1101/gad.1989510
|
| PII |
gad.1989510
|
| PMID |
20966047
|
| PMC |
PMC2975925
|
| MeSH |
Animals
Cell Line
Cell Nucleus / metabolism
Cytoplasm / metabolism
DNA Transposable Elements / physiology
Drosophila Proteins / metabolism*
Drosophila melanogaster / metabolism
Drosophila melanogaster / physiology*
Endoribonucleases / metabolism
Protein Transport
RNA Helicases / metabolism*
RNA, Small Interfering / biosynthesis*
|
| IF |
9.527
|
| リソース情報 |
| ショウジョウバエ |
10011Ab-1
10012Ab-1 |
