RRC ID 77416
Author Saito K, Ishizu H, Komai M, Kotani H, Kawamura Y, Nishida KM, Siomi H, Siomi MC.
Title Roles for the Yb body components Armitage and Yb in primary piRNA biogenesis in Drosophila.
Journal Genes Dev
Abstract PIWI-interacting RNAs (piRNAs) protect genome integrity from transposons. In Drosophila ovarian somas, primary piRNAs are produced and loaded onto Piwi. Here, we describe roles for the cytoplasmic Yb body components Armitage and Yb in somatic primary piRNA biogenesis. Armitage binds to Piwi and is required for localizing Piwi into Yb bodies. Without Armitage or Yb, Piwi is freed from the piRNAs and does not enter the nucleus. Thus, piRNA loading is required for Piwi nuclear entry. We propose that a functional Piwi-piRNA complex is formed and inspected in Yb bodies before its nuclear entry to exert transposon silencing.
Volume 24(22)
Pages 2493-8
Published 2010-11-15
DOI 10.1101/gad.1989510
PII gad.1989510
PMID 20966047
PMC PMC2975925
MeSH Animals Cell Line Cell Nucleus / metabolism Cytoplasm / metabolism DNA Transposable Elements / physiology Drosophila Proteins / metabolism* Drosophila melanogaster / metabolism Drosophila melanogaster / physiology* Endoribonucleases / metabolism Protein Transport RNA Helicases / metabolism* RNA, Small Interfering / biosynthesis*
IF 9.527
Resource
Drosophila 10011Ab-1 10012Ab-1 10015Ab-1 10016Ab-1