| RRC ID |
77422
|
| Author |
Akimoto AM, Takarada T, Maeda M.
|
| Title |
Preparation of cell-culturing glass surfaces that release branched polyethyleneimine triggered by thiol-disulfide exchange.
|
| Journal |
Colloids Surf B Biointerfaces
|
| Abstract |
To develop a chemical stimulus-responsive substrate for culturing cells, polyethyleneimine (PEI) having a pyridyl disulfide moiety was attached via disulfide linkages to a glass coverslip modified with a silane coupling agent having a thiol group. The surface modification was confirmed by X-ray photoelectron spectroscopy and zeta potential analysis. The obtained surface exhibited sufficiently high cell adhesiveness. Zeta potential measurements revealed that the PEI derivatives were released from the surface through thiol-disulfide exchange when the modified glass coverslip was immersed in a neutral pH buffer containing cysteine. The cell viability assay demonstrated that this chemical stimulus was substantially nontoxic to 293T cells. Because PEI is a widely used transfection reagent, this functional glass coverslip would be potentially useful as an experimental platform for reverse transfection.
|
| Volume |
103
|
| Pages |
360-5
|
| Published |
2013-3-1
|
| DOI |
10.1016/j.colsurfb.2012.10.031
|
| PII |
S0927-7765(12)00588-7
|
| PMID |
23261556
|
| MeSH |
Cell Culture Techniques / methods*
Cell Survival
Cells, Cultured
Disulfides / chemistry*
Glass / chemistry*
Humans
Polyethyleneimine / chemistry*
Static Electricity
Sulfhydryl Compounds / chemistry*
Surface Properties
|
| IF |
4.389
|
| Resource |
| Human and Animal Cells |
293T(RCB2202) |
