RRC ID 77460
Author Yuko Watanabe, Eri Katsumura, Tatsuki Domon, Yuta Ishikawa, Rina Oguri, Minami Takashima, Qi Meng, Masato Kinoshita, Hisashi Hashimoto, Kiyotaka Hitomi
Title Establishment of transgenic epithelium-specific Cre-recombinase driving medaka (Oryzias latipes) by homology repair mediated knock-in
Journal Bioscience, Biotechnology, and Biochemistry
Abstract ABSTRACT Deletion of gene expression in the target tissues and cells is an effective strategy for elucidating the physiological functions of the protein of interest. For tissue-specific and/or inducible gene deletion, the Cre-loxP system has been widely used in various model organisms including medaka (Oryzias latipes). The epithelium is the key tissue, locating at the outermost area and playing a role in barrier to external stimuli. Despite a large genetic toolbox developed in medaka, there is no available Cre-driver line that works in an epithelium-specific manner. Here, we established epithelium-specific Cre-driver lines in medaka using a homology-directed repair mediated knock-in approach with CRISPR/Cas9, targeting each of periplakin and keratin genes. We show that Cre-recombinase is expressed exclusively in the epithelium in the knock-in lines and that it efficiently and specifically induces recombination in the tissues. These Cre-driver lines are useful for studying the functions of proteins expressed in the epithelium.
Volume 87
Pages 1285-1294
Published 2023-10-25
DOI 10.1093/bbb/zbad116
PMID 37607777
MeSH Animals Animals, Genetically Modified Integrases / genetics Integrases / metabolism Oryzias* / genetics
IF 1.516
Medaka OK-Cab (MT830) d-rR-Tg(beta-actin-loxP-DsRed2-loxP-GFP) (TG861)