| RRC ID |
77460
|
| 著者 |
Yuko Watanabe, Eri Katsumura, Tatsuki Domon, Yuta Ishikawa, Rina Oguri, Minami Takashima, Qi Meng, Masato Kinoshita, Hisashi Hashimoto, Kiyotaka Hitomi
|
| タイトル |
Establishment of transgenic epithelium-specific Cre-recombinase driving medaka (Oryzias latipes) by homology repair mediated knock-in
|
| ジャーナル |
Bioscience, Biotechnology, and Biochemistry
|
| Abstract |
ABSTRACT
Deletion of gene expression in the target tissues and cells is an effective strategy for elucidating the physiological functions of the protein of interest. For tissue-specific and/or inducible gene deletion, the Cre-loxP system has been widely used in various model organisms including medaka (Oryzias latipes). The epithelium is the key tissue, locating at the outermost area and playing a role in barrier to external stimuli. Despite a large genetic toolbox developed in medaka, there is no available Cre-driver line that works in an epithelium-specific manner. Here, we established epithelium-specific Cre-driver lines in medaka using a homology-directed repair mediated knock-in approach with CRISPR/Cas9, targeting each of periplakin and keratin genes. We show that Cre-recombinase is expressed exclusively in the epithelium in the knock-in lines and that it efficiently and specifically induces recombination in the tissues. These Cre-driver lines are useful for studying the functions of proteins expressed in the epithelium.
|
| 巻・号 |
87
|
| ページ |
1285-1294
|
| 公開日 |
2023-10-25
|
| DOI |
10.1093/bbb/zbad116
|
| PMID |
37607777
|
| MeSH |
Animals
Animals, Genetically Modified
Integrases / genetics
Integrases / metabolism
Oryzias* / genetics
|
| IF |
1.516
|
| リソース情報 |
| メダカ |
OK-Cab (MT830)
d-rR-Tg(beta-actin-loxP-DsRed2-loxP-GFP) (TG861) |
