論文 - 詳細
| RRC ID | 77517 |
|---|---|
| 著者 | Xiao H, Hasegawa T, Isobe K. |
| タイトル | p300 collaborates with Sp1 and Sp3 in p21(waf1/cip1) promoter activation induced by histone deacetylase inhibitor. |
| ジャーナル | J Biol Chem |
| Abstract |
We have reported that histone acetylation induced by trichostatin A (TSA) promotes p21(waf1/cip1) (p21) expression, the GC-box located just upstream of TATA box was responsible for TSA-induced promoter activation, and both Sp1 and Sp3 were the working activator of this GC-box. To understand the molecular pathway from histone acetylation to this Sp1 family factors-mediated promoter activation, we investigated the function of p300, one of the histone acetyltransferase, in the present work. The evidence supporting the linkage between p300 and TSA-induced p21 promoter activation were realized from the following findings: 1) cotransfection of p300 elevated p21 promoter activity, and this elevation was dependent on TSA-responsive GC-box; 2) TSA-induced promoter activation was blocked by the introduction of p300 dominant-negative mutant into cells; and 3) Sp1- or Sp3-mediated activation was also suppressed by this p300 dominant-negative mutant. Our data also suggested that p300 collaborates with Sp1 in a way which is different from that when p300 collaborates with p53 in p21 transcription. |
| 巻・号 | 275(2) |
| ページ | 1371-6 |
| 公開日 | 2000-1-14 |
| DOI | 10.1074/jbc.275.2.1371 |
| PII | S0021-9258(18)31259-6 |
| PMID | 10625687 |
| MeSH | Animals COS Cells Cyclin-Dependent Kinase Inhibitor p21 Cyclins / genetics* DNA-Binding Proteins / metabolism* Enzyme Inhibitors / metabolism Enzyme Inhibitors / pharmacology* Gene Expression Regulation Genes, Reporter HeLa Cells Histone Deacetylase Inhibitors* Humans Hydroxamic Acids / pharmacology* Luciferases / genetics Nuclear Proteins / metabolism* Promoter Regions, Genetic* Recombinant Fusion Proteins / biosynthesis Sp1 Transcription Factor / metabolism* Sp3 Transcription Factor Trans-Activators / metabolism* Transcription Factors / metabolism* Transfection |
| IF | 4.238 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | COS-1(RCB0143) |